| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 451,725 | C→G | 30.9% | L103L (CTG→CTC) | kdpA ← | potassium translocating ATPase, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 451,725 | 0 | C | G | 30.9% | 116.7 / 66.6 | 97 | L103L (CTG→CTC) | kdpA | potassium translocating ATPase, subunit A |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base G (23/7); total (53/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAA > minE/451659‑451793 | gCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAg > 1:2344205/1‑69 (MQ=255) cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc < 1:1577947/69‑1 (MQ=255) cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc < 1:80522/69‑1 (MQ=255) cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc < 1:335408/69‑1 (MQ=255) cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc < 1:2713434/69‑1 (MQ=255) cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc < 1:3384919/69‑1 (MQ=255) cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc < 1:3345661/69‑1 (MQ=255) gTTTCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc < 1:1299379/62‑1 (MQ=255) gTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATcc < 1:724761/69‑1 (MQ=255) gTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATcc < 1:1371061/69‑1 (MQ=255) gTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATcc > 1:634185/1‑69 (MQ=255) gTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATcc > 1:3067735/1‑69 (MQ=255) ttCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATccc > 1:1290318/1‑68 (MQ=255) ttCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATccc > 1:1807783/1‑68 (MQ=255) ttCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATccc > 1:2726726/1‑68 (MQ=255) ttCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATccc > 1:1964040/1‑68 (MQ=255) ttCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATccc > 1:2425639/1‑68 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2343177/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:3267730/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:1942945/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2476641/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2606397/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:3134601/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2735297/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2737932/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2805821/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2806931/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2816069/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2820109/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:3214660/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:3095959/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:158874/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:779736/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:772293/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:1475109/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:1269005/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:139578/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:851504/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:1793545/1‑59 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATT‑Agcgc > 1:2466374/1‑58 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc < 1:1291993/69‑1 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc < 1:849708/69‑1 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc < 1:2116362/69‑1 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc > 1:296466/1‑69 (MQ=255) tCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc > 1:121223/1‑69 (MQ=255) tCACCGCTATAAGATTGCAAGTTGGTGTTAGTGACAAAGCTGACGGCGGTATTGAgcgc > 1:2229700/1‑59 (MQ=255) cTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:1284649/69‑1 (MQ=255) cTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:3258410/69‑1 (MQ=255) cTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:1956415/69‑1 (MQ=255) cTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:1627980/69‑1 (MQ=255) cTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:699022/69‑1 (MQ=255) cTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:2324143/69‑1 (MQ=255) cTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:2240347/69‑1 (MQ=255) cTATAACATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:1246540/69‑1 (MQ=255) aaGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:1688248/65‑1 (MQ=255) aGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc < 1:1853787/56‑1 (MQ=255) tGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg > 1:2500505/1‑69 (MQ=255) tGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg > 1:1882251/1‑69 (MQ=255) tGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg > 1:345354/1‑69 (MQ=255) tGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg > 1:2428071/1‑69 (MQ=255) ttGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa < 1:1330983/69‑1 (MQ=255) ttGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa < 1:1681746/69‑1 (MQ=255) ttGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa < 1:3247816/69‑1 (MQ=255) ttGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa < 1:923869/69‑1 (MQ=255) ttGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa < 1:3112563/69‑1 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGGCAGCCCTGGCAACTGCTGTGGATTAAg > 1:3014776/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:386164/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:81630/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:3208618/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:94975/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:1119906/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:295441/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:1302430/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:1469992/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:2321017/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:1538014/1‑69 (MQ=255) ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:2224943/1‑69 (MQ=255) ggTGACAAAGCTGACGCCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg > 1:515123/1‑69 (MQ=255) aCAAAGCTGACGGCGGTATTCAGCGCCAGATCCCAc < 1:477904/36‑1 (MQ=255) aaGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa < 1:2968357/69‑1 (MQ=255) aaGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa < 1:3061497/69‑1 (MQ=255) gCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa < 1:972410/67‑1 (MQ=255) gCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa < 1:962712/67‑1 (MQ=255) gCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa < 1:1395270/67‑1 (MQ=255) gCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa < 1:2635625/67‑1 (MQ=255) ggTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa < 1:1590768/58‑1 (MQ=255) ggTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGa > 1:231310/1‑67 (MQ=255) ggTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGa > 1:538407/1‑67 (MQ=255) ggTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGa > 1:74766/1‑67 (MQ=255) ttCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc < 1:2204392/69‑1 (MQ=255) ttCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc < 1:1762988/69‑1 (MQ=255) ttCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc < 1:3388861/69‑1 (MQ=255) ttCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc < 1:1472389/69‑1 (MQ=255) ttCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc < 1:2348749/69‑1 (MQ=255) ttCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc < 1:2466029/69‑1 (MQ=255) ttCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc < 1:1208181/69‑1 (MQ=255) ttCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc < 1:2880739/69‑1 (MQ=255) cAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCaa > 1:1852903/1‑69 (MQ=255) cAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCaa > 1:3335378/1‑69 (MQ=255) cAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCaa > 1:1001233/1‑69 (MQ=255) | GCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAA > minE/451659‑451793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |