| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 452,550 | G→A | 34.3% | R172H (CGC→CAC) | phr → | deoxyribodipyrimidine photolyase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 452,550 | 0 | G | A | 34.3% | 46.8 / 33.3 | 64 | R172H (CGC→CAC) | phr | deoxyribodipyrimidine photolyase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base G (22/20); new base A (15/7); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAG > minE/452483‑452616 | tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:1129515/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:1145737/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:945079/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:701079/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:1204091/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:415312/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:2187536/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:2178370/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTgcg > 1:1928257/1‑68 (MQ=255)tCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGACGCCGGAGTGCGTCGCTgcg > 1:998948/1‑68 (MQ=255) tttACGCCTTTTAAGAATGCCTGGCTGAAACGGGTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:2938102/1‑68 (MQ=255) tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:1123235/1‑68 (MQ=255) tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:490187/1‑68 (MQ=255) tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:445826/1‑68 (MQ=255) tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:337611/1‑68 (MQ=255) tttACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGcc > 1:134946/1‑68 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAg > 1:2717854/1‑68 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:181935/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:2603392/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:2605418/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:265014/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:447106/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:857962/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:2808036/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:850829/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:524756/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:3177408/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:1557723/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:985546/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:468708/1‑69 (MQ=255) gCCTTTTAAAAACGCATGGTTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCACCAAAAGt > 1:489261/1‑69 (MQ=255) gCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGa < 1:2918476/69‑1 (MQ=255) tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:60730/68‑1 (MQ=255) tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:329972/68‑1 (MQ=255) tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:608943/68‑1 (MQ=255) tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:215564/68‑1 (MQ=255) tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:2194774/68‑1 (MQ=255) tGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATc < 1:881025/68‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:1303224/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:94977/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:1185179/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:1171606/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:2296814/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:24416/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:2671803/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:415627/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGa < 1:2560149/69‑1 (MQ=255) ggCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGAGGATCGa < 1:2097339/69‑1 (MQ=255) gAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATaga < 1:870012/69‑1 (MQ=255) gAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATaga < 1:1468952/69‑1 (MQ=255) gAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATaga < 1:2519815/69‑1 (MQ=255) gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:521613/1‑66 (MQ=255) gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:612876/1‑66 (MQ=255) gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2737384/1‑66 (MQ=255) gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2660126/1‑66 (MQ=255) gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2542580/1‑66 (MQ=255) gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2519245/1‑66 (MQ=255) gATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGccatccat > 1:2031065/1‑66 (MQ=255) ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:798798/1‑69 (MQ=255) ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:1023626/1‑69 (MQ=255) ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:171342/1‑69 (MQ=255) ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:316003/1‑69 (MQ=255) ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:2482986/1‑69 (MQ=255) ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:2247162/1‑69 (MQ=255) ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:2243116/1‑69 (MQ=255) ccGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTg > 1:2131850/1‑69 (MQ=255) gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGATCCCGCGCCATCCATTACGCTGAATTATc < 1:347568/69‑1 (MQ=255) gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:1356270/69‑1 (MQ=255) gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:3322340/69‑1 (MQ=255) gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:3016770/69‑1 (MQ=255) gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:861349/69‑1 (MQ=255) gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:2411340/69‑1 (MQ=255) gCGTCGCTGCACCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCGCGCCATCCATTACGCTGAATTATc < 1:1967639/69‑1 (MQ=255) cGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATcc > 1:2829707/1‑69 (MQ=255) gcgcCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAg < 1:1255017/69‑1 (MQ=255) gcgcCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAg < 1:3282142/69‑1 (MQ=255) gcgcCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAg < 1:1499769/69‑1 (MQ=255) | TCTTTACGCCTTTTAAGAATGCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAG > minE/452483‑452616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |