| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 53,762 | A→G | 36.2% | D342D (GAT→GAC) | imp ← | exported protein required for envelope biosynthesis and integrity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 53,762 | 0 | A | G | 36.2% | 97.5 / 94.0 | 149 | D342D (GAT→GAC) | imp | exported protein required for envelope biosynthesis and integrity |
| Reads supporting (aligned to +/- strand): ref base A (43/52); new base G (22/32); total (65/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
CCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACAC > minE/53695‑53818 | ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:2183345/69‑1 (MQ=255)ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:3430738/69‑1 (MQ=255)ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:2689377/69‑1 (MQ=255)ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:3054471/69‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:986253/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:215365/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:2077264/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:2073292/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:2034162/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:2288229/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:1962329/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:2313863/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:248832/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:2631282/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:15113/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:754405/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:591515/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:137801/1‑68 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:1496126/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:2499399/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:1772788/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:1672675/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:3125072/1‑69 (MQ=255) gAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAgg < 1:1742567/69‑1 (MQ=255) gAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAgg < 1:3035443/69‑1 (MQ=255) gAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAgg < 1:343134/69‑1 (MQ=255) gttgcgtAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGAc < 1:3285998/68‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1017530/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:697937/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:65872/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3144948/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2270104/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2311756/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:335326/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3234362/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3179502/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3203727/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2828964/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2817122/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1125598/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:192655/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2166252/69‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1287654/68‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:631914/68‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1171011/68‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:102086/68‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3190794/68‑1 (MQ=255) cgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:975568/67‑1 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCTTTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCgc > 1:620848/1‑67 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1515753/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1868940/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:696079/1‑69 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:38647/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:3126507/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:529168/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1245649/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:293543/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1193483/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:3378098/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1008402/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:2594279/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:776013/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:2523425/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:200293/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGGCGACGt < 1:2380714/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTACTCGACGt < 1:2571563/69‑1 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:1458305/1‑68 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:193140/1‑68 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:1199632/1‑68 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:899636/1‑68 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTTGGATCGCTGACTTTg > 1:834786/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAgg > 1:662616/1‑39 (MQ=38) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:473771/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:387248/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:841725/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1452030/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2022502/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:3195544/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2012637/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1919040/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1569004/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2820069/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1770557/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2765329/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTCAAGTAGCTAGGATCGCTGACTTTg > 1:2935780/1‑52 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:132714/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:896301/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:2525945/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:2545619/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:2698557/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:563198/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGAGCGCTGACCTTGGTGTAGTCGACGTTGa < 1:3141628/68‑1 (MQ=255) ccGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGc < 1:2608109/69‑1 (MQ=255) ccGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGc < 1:2248658/69‑1 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1836879/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1606981/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1429936/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1378874/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1316477/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:2069906/1‑69 (MQ=255) | CCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACAC > minE/53695‑53818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |