Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 454,683 C→T 30.3% P134P (CCG→CCA)  ybgH ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE454,6830CT30.3% 127.2 / 60.7 129P134P (CCG→CCA) ybgHpredicted transporter
Reads supporting (aligned to +/- strand):  ref base C (72/17);  new base T (27/12);  total (100/29)
Fisher's exact test for biased strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 129 total aligned reads displayed.

AGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGC  >  minE/454620‑454741
                                                               |                                                          
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGAt                                                        >  1:1280166/1‑68 (MQ=255)
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:2313998/1‑69 (MQ=255)
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:2362797/1‑69 (MQ=255)
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:2552536/1‑69 (MQ=255)
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:2585229/1‑69 (MQ=255)
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:788778/1‑69 (MQ=255)
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:161971/1‑69 (MQ=255)
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:3197438/1‑69 (MQ=255)
aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:3416035/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCCTCACGACGCGGATCGGtt                                                   >  1:2233393/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                   >  1:1032394/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                   >  1:2599131/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                   >  1:1781932/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                   >  1:2663559/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                   >  1:2817700/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                   >  1:1415982/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                   >  1:1202219/1‑69 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                    >  1:3277830/1‑68 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                    >  1:106116/1‑68 (MQ=255)
    gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt                                                    >  1:968414/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGtt                                                   >  1:573054/1‑66 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:1809961/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:2329167/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:2155047/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:200095/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:991805/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:2477028/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:1892178/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:1113934/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:815338/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:3370726/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:579820/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:1776562/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:2630007/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:2653877/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:449640/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:1598078/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:3009899/1‑68 (MQ=255)
       ataataGACCCCACGTTACCCGCAGCATACATCAGCGAGAAACCGCCATCACGACGTGGATCGGTTgg                                                 >  1:1033372/1‑68 (MQ=255)
                cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATc                                                       >  1:221351/1‑53 (MQ=255)
                cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                       >  1:514570/1‑69 (MQ=255)
                cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                       >  1:1372277/1‑69 (MQ=255)
                      ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                 >  1:1569141/1‑69 (MQ=255)
                           cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                             <  1:172243/68‑1 (MQ=255)
                           cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                             <  1:3118277/68‑1 (MQ=255)
                           cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                             <  1:321209/68‑1 (MQ=255)
                           cgccgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTGGGCTCATACAGCTCGCCGAGCa                             <  1:2843583/68‑1 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                    >  1:865530/1‑68 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:276323/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:1541306/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:3199758/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:2298298/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:1522422/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:2954836/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:2933241/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:2162980/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:520298/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:520784/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:579050/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:2507799/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:1187526/1‑69 (MQ=255)
                                    cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:1714803/1‑68 (MQ=255)
                                        aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:1944063/1‑65 (MQ=255)
                                        aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:3024298/1‑65 (MQ=255)
                                              aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa         <  1:434044/69‑1 (MQ=255)
                                              aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa         <  1:98680/69‑1 (MQ=255)
                                              aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa         <  1:2280073/69‑1 (MQ=255)
                                                aCCGCCATCACGACGTGGATCGGTTGGCTCATACAGCTCGCCGAGc                              <  1:1085881/46‑1 (MQ=255)
                                                aCCGCCATCACGACGTGGATCGGTTGGCTCATACAGCTCGCCGAGc                              <  1:1394210/46‑1 (MQ=255)
                                                aCCGCCATCACGACGTGGATCGGTTGGCTCATACAGCTCGCCGAGc                              <  1:3395147/46‑1 (MQ=255)
                                                aCCGCCATCACGACGTGGATCGGTTGGCTCATACAGCTCGCCGAGc                              <  1:222152/46‑1 (MQ=255)
                                                aCCGCCATCACGACGTGGATCGGTTGGCTCATACAGCTCGCCGAGc                              <  1:703688/46‑1 (MQ=255)
                                                aCCGCCATCACGACGTGGATCGGTTGGCTCATACAGCTCGCCGAGc                              <  1:1686881/46‑1 (MQ=255)
                                                aCCGCCATCACGACGTGGATCGGTTGGCTCATACAGCTCGCCGAGc                              <  1:1881805/46‑1 (MQ=255)
                                                aCCGCCATCACGACGTGGATCGGTTGGCTCATACAGCTCGCCGAGc                              <  1:1728268/46‑1 (MQ=255)
                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  <  1:216329/69‑1 (MQ=255)
                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  <  1:1127504/69‑1 (MQ=255)
                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  <  1:708281/69‑1 (MQ=255)
                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  <  1:1992534/69‑1 (MQ=255)
                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  <  1:296908/69‑1 (MQ=255)
                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  <  1:2857163/69‑1 (MQ=255)
                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  <  1:2477527/69‑1 (MQ=255)
                                                     cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  <  1:3117186/69‑1 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                             >  1:1818145/1‑38 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                   >  1:506610/1‑48 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:2728020/1‑63 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:254465/1‑63 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:1510367/1‑63 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:1150973/1‑63 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:832799/1‑63 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:843444/1‑63 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:1995433/1‑63 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:3045709/1‑63 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  >  1:2738404/1‑65 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  >  1:3368369/1‑65 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  >  1:2474443/1‑65 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  >  1:1096704/1‑65 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTCATACAGCTCGACGAGCAGACAGCTTACGTTAGATTTAAACAGGc  >  1:1527919/1‑65 (MQ=255)
                                                         acgacgCGGATCGGTTGGCTAATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGc  >  1:3273958/1‑65 (MQ=255)
                                                         acgacgAGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAg    >  1:441979/1‑63 (MQ=255)
                                                               |                                                          
AGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGC  >  minE/454620‑454741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑