| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 53,789 | C→T | 26.2% | K333K (AAG→AAA) | imp ← | exported protein required for envelope biosynthesis and integrity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 53,789 | 0 | C | T | 26.2% | 137.2 / 43.5 | 114 | K333K (AAG→AAA) | imp | exported protein required for envelope biosynthesis and integrity |
| Reads supporting (aligned to +/- strand): ref base C (52/32); new base T (19/11); total (71/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01 | |||||||||||
Only 100 of 146 total aligned reads displayed.
TGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGA > minE/53723‑53850 | tgtgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2647594/66‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3234362/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2817122/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2977069/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3144948/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2313475/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2311756/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2270104/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3203727/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:192655/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3223025/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3380199/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:145483/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:65872/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1017530/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:697937/69‑1 (MQ=255)tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:869080/69‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:631914/68‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:102086/68‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1171011/68‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3190794/68‑1 (MQ=255) gcgtAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTg > 1:3060091/1‑69 (MQ=255) gcgtAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTg > 1:220889/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCTTTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCgc > 1:620848/1‑67 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:2014728/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:3128899/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1216823/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:2165881/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:319390/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1930901/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:696079/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1515753/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1868940/1‑69 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1193483/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1008402/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:2594279/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:529168/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:2523425/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1607940/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:38647/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1757760/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:3378098/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1245649/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:200293/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTACTCGACGt < 1:2571563/69‑1 (MQ=255) aCTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1387042/68‑1 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:1199632/1‑68 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:899636/1‑68 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:1965963/1‑68 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:841725/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:387248/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2765329/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2820069/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2022502/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:191660/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1770557/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1337653/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1569004/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1507414/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1452030/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTCAAGTAGCTAGGATCGCTGACTTTg > 1:2935780/1‑52 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:2525945/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:563198/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:2698557/68‑1 (MQ=255) ccGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGc < 1:2248658/69‑1 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt > 1:806029/1‑56 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1378874/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1316477/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1606981/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:2069906/1‑69 (MQ=255) aGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAg < 1:1163199/68‑1 (MQ=255) aTCGCTGACTTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGt < 1:3179292/68‑1 (MQ=255) aTCGCTGACTTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGt < 1:3103472/68‑1 (MQ=255) aTCGCTGACTTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGt < 1:2837596/68‑1 (MQ=255) aTCGCTGACTTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGt < 1:2783583/68‑1 (MQ=255) aTCGCTGACTTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGt < 1:1289935/68‑1 (MQ=255) aTCGCTGACTTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGt < 1:795842/68‑1 (MQ=255) aTCGCTGACTTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGt < 1:443751/68‑1 (MQ=255) aTCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAg < 1:1596064/67‑1 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTcc > 1:329070/1‑64 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTcc > 1:2405686/1‑64 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTa > 1:53536/1‑68 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAg < 1:1712932/69‑1 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAg > 1:1822056/1‑69 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAg > 1:926737/1‑69 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAg < 1:293371/69‑1 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAg > 1:2860360/1‑69 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAg > 1:2834852/1‑69 (MQ=255) tCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAg > 1:796733/1‑69 (MQ=255) cGCTGAGCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:439484/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:342300/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:983007/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:986585/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:821875/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:575099/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:1702744/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:2396501/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:313851/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:3006243/1‑69 (MQ=255) cGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGa > 1:2776526/1‑69 (MQ=255) | TGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGA > minE/53723‑53850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |