| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 461,006 | T→G | 100% | intergenic (‑83/‑626) | gltA ← / → sdhC | citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 461,006 | 0 | T | G | 88.7% | 75.4 / 1.1 | 35 | intergenic (‑83/‑626) | gltA/sdhC | citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base G (31/0); total (32/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGG > minE/460940‑461071 | gcCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt < 1:1057568/69‑1 (MQ=255)gcCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt < 1:2560284/69‑1 (MQ=255)gcCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt < 1:1800697/69‑1 (MQ=255) ccTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt < 1:3103989/68‑1 (MQ=255) tAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc > 1:1774753/1‑69 (MQ=255) aaCTTAAATTTGCCTTCGCACATCAACCTGTCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaaa < 1:1138715/69‑1 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:932892/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:535493/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:3339949/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:2596151/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:2457237/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:2119353/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:2044344/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:1705211/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:1561326/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:1400806/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAag > 1:1226270/1‑69 (MQ=255) aCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTGTTATTTGGCTCGACGCTCTGTGAAag > 1:1469693/1‑69 (MQ=255) cacaTCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTggg < 1:2253258/67‑1 (MQ=255) tttACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcg < 1:877105/69‑1 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAagag > 1:2486850/1‑37 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTggg > 1:3059433/1‑64 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:2921928/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:1646170/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:1233648/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:3386838/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:486599/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:1214975/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:859327/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:882058/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:2689225/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:2324409/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:2185015/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:1546912/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:2094867/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcg > 1:378302/1‑66 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCt > 1:3124423/1‑68 (MQ=255) aCCCGTTTGTTATTTGGCTCGCCGCCCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:2883218/1‑67 (MQ=255) aCCCGTTTGTTATTTGGCTCGCAGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGgcgc > 1:925362/1‑67 (MQ=255) ttttATTTGGCTCGCCGCTCTGTTAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAgg > 1:1011352/1‑67 (MQ=255) ttttATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAgg > 1:2688903/1‑67 (MQ=255) ttttATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAgg > 1:2635269/1‑67 (MQ=255) ttttATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAgg > 1:25063/1‑67 (MQ=255) ttttATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAgg > 1:2457807/1‑67 (MQ=255) ttttATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAgg > 1:1501512/1‑67 (MQ=255) ttttATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAgg > 1:648079/1‑67 (MQ=255) | GCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGG > minE/460940‑461071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |