| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 464,737 | T→C | 40.8% | S198S (AGT→AGC) | sdhB → | succinate dehydrogenase, FeS subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 464,737 | 0 | T | C | 40.8% | 37.3 / 71.1 | 98 | S198S (AGT→AGC) | sdhB | succinate dehydrogenase, FeS subunit |
| Reads supporting (aligned to +/- strand): ref base T (42/16); new base C (24/16); total (66/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
TAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTG > minE/464670‑464786 | tAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGt < 1:1087369/68‑1 (MQ=255)tAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGt < 1:367292/68‑1 (MQ=255)tAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGt < 1:456298/68‑1 (MQ=255)tAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGt < 1:681024/68‑1 (MQ=255) tatCGTTTCCTGATTGATAGCCGTTATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTc > 1:1117033/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:1474008/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:3410208/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:1480532/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:2456411/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:1579361/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:158897/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:2433016/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:2409056/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:1706727/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:2350096/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:2334227/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:1916246/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:1406768/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:1388786/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:3287460/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:479335/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:1335239/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:487183/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:110484/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:681460/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:736108/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:907955/1‑69 (MQ=255) tCGTTTCCTGATTGATAGCCGTGATACAGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAg > 1:884486/1‑69 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCAtt > 1:1043104/1‑64 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGc > 1:2697698/1‑68 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:1380973/1‑69 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:2868837/1‑69 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:2712403/1‑69 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:723965/1‑69 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:3060049/1‑69 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:884478/1‑69 (MQ=255) gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:1869110/1‑69 (MQ=255) ttCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTa < 1:2366740/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGTTTTGAGCGATGCATTCAGTGTATTCCg < 1:3080154/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCAt > 1:2021751/1‑56 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTcc > 1:675847/1‑68 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:3065800/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:664723/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1084930/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:2898700/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1337722/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:2905543/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:1765616/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:679753/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:3093714/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:1021211/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:3347036/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1100571/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1342559/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1338593/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:120843/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:2042867/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:2789528/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:737234/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1884480/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:2330642/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:81857/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:1846458/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:2435890/69‑1 (MQ=255) tgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCt > 1:1751092/1‑68 (MQ=255) tgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCt > 1:168392/1‑68 (MQ=255) tgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCt > 1:3053589/1‑68 (MQ=255) tgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCt > 1:635211/1‑68 (MQ=255) gatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGtc > 1:1306879/1‑59 (MQ=255) gatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTa > 1:2668222/1‑60 (MQ=255) aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATg < 1:1551299/69‑1 (MQ=255) aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATg < 1:2470856/69‑1 (MQ=255) aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATg < 1:810780/69‑1 (MQ=255) aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATg < 1:342022/69‑1 (MQ=255) ccGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGa > 1:562543/1‑69 (MQ=255) ccGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGa > 1:2811198/1‑69 (MQ=255) ccGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGa > 1:449132/1‑69 (MQ=255) cGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1714586/51‑1 (MQ=255) gaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTg < 1:2824989/69‑1 (MQ=255) gaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTg < 1:2313843/69‑1 (MQ=255) gaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTg < 1:3079215/69‑1 (MQ=255) gaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTg < 1:2926174/69‑1 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGCGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTg > 1:2781731/1‑68 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1078231/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:823325/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:3038218/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:468186/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:2901185/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:2886572/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:2785528/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1633795/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1748919/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1855452/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1782189/1‑69 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:445293/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:1362683/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:683344/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:2540669/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:2275934/69‑1 (MQ=255) cgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:495882/67‑1 (MQ=255) | TAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTG > minE/464670‑464786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |