| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 464,749 | C→T | 54.2% | S202S (AGC→AGT) | sdhB → | succinate dehydrogenase, FeS subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 464,749 | 0 | C | T | 54.2% | 6.9 / 59.7 | 72 | S202S (AGC→AGT) | sdhB | succinate dehydrogenase, FeS subunit |
| Reads supporting (aligned to +/- strand): ref base C (16/17); new base T (23/16); total (39/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTG > minE/464682‑464786 | gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGc > 1:2697698/1‑68 (MQ=255)gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:3060049/1‑69 (MQ=255)gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:1869110/1‑69 (MQ=255)gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:723965/1‑69 (MQ=255)gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:2868837/1‑69 (MQ=255)gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:884478/1‑69 (MQ=255)gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:1380973/1‑69 (MQ=255)gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCg > 1:2712403/1‑69 (MQ=255) ttCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTa < 1:2366740/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGTTTTGAGCGATGCATTCAGTGTATTCCg < 1:3080154/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTcc > 1:675847/1‑68 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:2042867/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:3093714/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:679753/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:2330642/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:2435890/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:664723/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:1021211/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:2789528/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:2898700/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:2905543/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:3347036/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:3065800/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:1846458/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:99595/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1084930/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1100571/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:120843/1‑69 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1337722/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1338593/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1342559/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:81857/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1884480/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:737234/69‑1 (MQ=255) gatcgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg > 1:1765616/1‑69 (MQ=255) tgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCt > 1:168392/1‑68 (MQ=255) tgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCt > 1:3053589/1‑68 (MQ=255) tgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCt > 1:1751092/1‑68 (MQ=255) tgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCt > 1:635211/1‑68 (MQ=255) gatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGtc > 1:1306879/1‑59 (MQ=255) gatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTa > 1:2668222/1‑60 (MQ=255) aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATg < 1:810780/69‑1 (MQ=255) aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATg < 1:2470856/69‑1 (MQ=255) aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATg < 1:342022/69‑1 (MQ=255) aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATg < 1:1551299/69‑1 (MQ=255) ccGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGa > 1:2811198/1‑69 (MQ=255) ccGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGa > 1:449132/1‑69 (MQ=255) ccGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGa > 1:562543/1‑69 (MQ=255) cGAGACTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCg < 1:1714586/51‑1 (MQ=255) gaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTg < 1:2824989/69‑1 (MQ=255) gaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTg < 1:2926174/69‑1 (MQ=255) gaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTg < 1:2313843/69‑1 (MQ=255) gaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTg < 1:3079215/69‑1 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGCGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTg > 1:2781731/1‑68 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:2901185/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1078231/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:823325/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1633795/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1748919/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1782189/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:1855452/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:2785528/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:468186/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:2886572/1‑69 (MQ=255) aCTGACAGCCGCCTCGACGGTTTGAGCGATGCATTCAGTGTATTCCGCTGTCACAGCATCATGAACTGc > 1:3038218/1‑69 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:2645746/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:2540669/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:2275934/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:683344/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:445293/69‑1 (MQ=255) gccgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:1362683/69‑1 (MQ=255) cgccTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:495882/67‑1 (MQ=255) cAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg < 1:2704365/41‑1 (MQ=255) | GTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTG > minE/464682‑464786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |