| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 465,500 | C→T | 34.7% | L114L (CTG→TTG) | sucA → | 2‑oxoglutarate decarboxylase, thiamin‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 465,500 | 0 | C | T | 34.7% | 87.8 / 65.2 | 115 | L114L (CTG→TTG) | sucA | 2‑oxoglutarate decarboxylase, thiamin‑requiring |
| Reads supporting (aligned to +/- strand): ref base C (58/17); new base T (26/14); total (84/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 115 total aligned reads displayed.
CCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTC > minE/465436‑465565 | ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:515493/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:1516778/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:2461262/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:509294/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:2424092/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:1801866/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:1996668/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:332733/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:2167290/1‑68 (MQ=255)ccTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACtgt > 1:2137732/1‑68 (MQ=255) tGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGgc > 1:659991/1‑69 (MQ=255) tGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGgc > 1:2100977/1‑69 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:416640/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:2294291/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:3081410/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:336617/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:1885418/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:499072/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:2403291/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:3037854/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:2909955/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:1687167/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:1613895/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:2746041/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:1559416/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:1005903/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:1160418/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:116272/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:652707/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:557195/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:1572761/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGATTGTGGCAGCAAGa > 1:551102/1‑68 (MQ=255) aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGAGATTGTGGCAGCAAGa > 1:2596059/1‑68 (MQ=255) tAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:2130152/69‑1 (MQ=255) tAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:2045846/69‑1 (MQ=255) aaCGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:2150516/68‑1 (MQ=255) aaCGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:622300/68‑1 (MQ=255) tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGgc > 1:2919535/1‑52 (MQ=255) tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGcc > 1:180483/1‑69 (MQ=255) tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGcc > 1:1328396/1‑69 (MQ=255) tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGcc > 1:921841/1‑69 (MQ=255) tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGcc > 1:2888725/1‑69 (MQ=255) tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGcc > 1:1146052/1‑69 (MQ=255) tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGcc > 1:1529743/1‑69 (MQ=255) tACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGc > 1:2385422/1‑68 (MQ=255) ccGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGa > 1:3139623/1‑69 (MQ=255) ccGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGa > 1:149335/1‑69 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:2853996/49‑1 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:1222114/49‑1 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:2578650/49‑1 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:1397659/49‑1 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:3166510/49‑1 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATaa < 1:1393744/49‑1 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGt > 1:1177358/1‑69 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGt > 1:1108355/1‑69 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGt > 1:220236/1‑69 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGt > 1:2130079/1‑69 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGt > 1:2624541/1‑69 (MQ=255) gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGCCAGCAAGATaa < 1:947759/49‑1 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACg > 1:2380397/1‑68 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACg > 1:1298730/1‑68 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGa > 1:1102613/1‑69 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGa > 1:1710229/1‑69 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGa > 1:1806983/1‑69 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGa > 1:2495682/1‑69 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGa > 1:2491649/1‑69 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGa > 1:2449767/1‑69 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGa > 1:1207943/1‑69 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGa > 1:1816655/1‑69 (MQ=255) tGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTCGATCCGTCTTTCCACGa > 1:3361090/1‑69 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:972360/1‑68 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:43889/1‑68 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:1575530/1‑68 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:3352278/1‑68 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:2230287/1‑68 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:1355929/1‑68 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:808644/1‑68 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:2989839/1‑68 (MQ=255) cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGAt > 1:2534425/1‑68 (MQ=255) tctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGa > 1:1368228/1‑68 (MQ=255) tctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGa > 1:1963066/1‑68 (MQ=255) tctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGa > 1:3389789/1‑68 (MQ=255) tctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGa > 1:3402648/1‑68 (MQ=255) tctcGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCAAGATCTGa > 1:1452714/1‑68 (MQ=255) tCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATTTGACCg < 1:435037/65‑1 (MQ=255) tCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCg < 1:900614/65‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:503965/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:2838949/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:34080/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:1370066/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:610608/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:2181296/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:638947/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:2286941/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:1332622/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:3092234/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:823185/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:1239082/69‑1 (MQ=255) ccGCTGGGATTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCa < 1:2313522/69‑1 (MQ=255) aCTGAGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTc < 1:270252/67‑1 (MQ=255) | CCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTC > minE/465436‑465565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |