| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 466,055 | A→T | 62.5% | M299L (ATG→TTG) | sucA → | 2‑oxoglutarate decarboxylase, thiamin‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 466,055 | 0 | A | T | 62.5% | 26.5 / 32.5 | 40 | M299L (ATG→TTG) | sucA | 2‑oxoglutarate decarboxylase, thiamin‑requiring |
| Reads supporting (aligned to +/- strand): ref base A (9/6); new base T (9/16); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCC > minE/465993‑466119 | tGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCt > 1:2731412/1‑69 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:1040075/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:811839/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:365473/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:340705/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:3163622/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:2678317/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:2607510/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:2025492/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:1956525/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:1919650/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:1823330/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:1124570/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:1588972/68‑1 (MQ=255) tgCCGGTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:1439760/68‑1 (MQ=255) gTAAACATAAAGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:518559/64‑1 (MQ=255) aaGAACACCTCGGCACGGGCGACGTGAAATACCACTTGGGCTTCTCGTCTGACtt < 1:508240/55‑1 (MQ=255) tCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:1016058/69‑1 (MQ=255) tCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:2305876/69‑1 (MQ=255) tCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:2398799/69‑1 (MQ=255) tCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:1817537/69‑1 (MQ=255) cGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:2850615/68‑1 (MQ=255) cGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:1134021/68‑1 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:1592980/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:18899/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:777993/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:725054/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:1678536/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:452163/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:431662/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:1172232/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:3043312/1‑69 (MQ=255) gTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGCTGGCGGCCTGGTGCACctggcgctggcg > 1:2721677/1‑69 (MQ=255) gTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctggcg > 1:2199556/1‑69 (MQ=255) gTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctggcg > 1:2609439/1‑69 (MQ=255) gTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctggcg > 1:1713100/1‑69 (MQ=255) ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc > 1:420028/1‑69 (MQ=255) ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc > 1:3264609/1‑69 (MQ=255) ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc > 1:2507961/1‑69 (MQ=255) ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc > 1:1210865/1‑69 (MQ=255) | TGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCC > minE/465993‑466119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |