| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 466,096 | G→T | 58.2% | V312V (GTG→GTT) | sucA → | 2‑oxoglutarate decarboxylase, thiamin‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 466,096 | 0 | G | T | 58.2% | 16.8 / 69.1 | 67 | V312V (GTG→GTT) | sucA | 2‑oxoglutarate decarboxylase, thiamin‑requiring |
| Reads supporting (aligned to +/- strand): ref base G (21/7); new base T (26/13); total (47/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAA > minE/466029‑466139 | tCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:1016058/69‑1 (MQ=255)tCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:2305876/69‑1 (MQ=255)tCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:1817537/69‑1 (MQ=255)tCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:2398799/69‑1 (MQ=255) cGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:1134021/68‑1 (MQ=255) cGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc < 1:2850615/68‑1 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:1592980/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:18899/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:1678536/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:3043312/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:431662/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:452163/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:1172232/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:725054/1‑69 (MQ=255) cGTGAAATACCACTTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTTCACctggcgctggc > 1:777993/1‑69 (MQ=255) gTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGCTGGCGGCCTGGTGCACctggcgctggcg > 1:2721677/1‑69 (MQ=255) gTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctggcg > 1:1713100/1‑69 (MQ=255) gTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctggcg > 1:2609439/1‑69 (MQ=255) gTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctggcg > 1:2199556/1‑69 (MQ=255) ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc > 1:3264609/1‑69 (MQ=255) ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc > 1:2507961/1‑69 (MQ=255) ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc > 1:420028/1‑69 (MQ=255) ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc > 1:1210865/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:193259/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:2020109/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:207510/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:1029185/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:732149/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:69925/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:2430280/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:126102/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:3275567/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:1263452/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:3214263/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:3073017/1‑69 (MQ=255) tGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtctc > 1:2744120/1‑69 (MQ=255) cGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgag < 1:420732/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGttt > 1:1967220/1‑43 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:176524/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:983054/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:2145320/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:766166/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:2206134/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:598518/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:474787/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:185342/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:436829/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:1229719/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:379651/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:2695297/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:2836548/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:256977/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgaga > 1:3149066/1‑62 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTgag > 1:189312/1‑61 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:1716535/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:892591/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:284795/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:110508/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:712057/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:710705/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:356952/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:1533484/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:2643846/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:1728920/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:1723305/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:247477/68‑1 (MQ=255) cTTCCAGACCGATGGCGGCCTGGTTCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTaa < 1:1005186/68‑1 (MQ=255) | TCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAA > minE/466029‑466139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |