| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 54,086 | C→T | 30.8% | K234K (AAG→AAA) | imp ← | exported protein required for envelope biosynthesis and integrity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 54,086 | 0 | C | T | 30.8% | 78.5 / 38.9 | 84 | K234K (AAG→AAA) | imp | exported protein required for envelope biosynthesis and integrity |
| Reads supporting (aligned to +/- strand): ref base C (26/32); new base T (5/21); total (31/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAA > minE/54023‑54143 | cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:160594/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:313040/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:2805350/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:1111194/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:729339/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:2379285/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:1294935/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:3040957/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:2901126/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:3001086/1‑69 (MQ=255)cATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc > 1:1448008/1‑69 (MQ=255) gcgcGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCggg < 1:1337146/68‑1 (MQ=255) gcgcGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCggg < 1:2601500/68‑1 (MQ=255) tGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTTGTGTACTTGGCGTTCGGGATCAAGa < 1:2814336/69‑1 (MQ=255) gTTCCAGTAATATGGCAGGTAGAACTCATAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGaa < 1:2460011/69‑1 (MQ=255) gTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGaa < 1:992725/69‑1 (MQ=255) gTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGaa < 1:2760891/69‑1 (MQ=255) gTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGaa < 1:812859/69‑1 (MQ=255) gTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGaa < 1:731245/69‑1 (MQ=255) gTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGaa < 1:1531723/69‑1 (MQ=255) gTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCaa > 1:1950849/1‑60 (MQ=255) gTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCaga > 1:1695386/1‑69 (MQ=255) gTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCaga > 1:2575683/1‑69 (MQ=255) gTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCag > 1:818643/1‑68 (MQ=255) gTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCa > 1:2981791/1‑67 (MQ=255) atatGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGc > 1:2205206/1‑68 (MQ=255) atatGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCg > 1:3211814/1‑69 (MQ=255) atatGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCg > 1:2598022/1‑69 (MQ=255) atatGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCg > 1:1508778/1‑69 (MQ=255) atatGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCg > 1:1723776/1‑69 (MQ=255) tGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACg < 1:1086374/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTTGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:2232267/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGTTCAAGAAACCAGAGCGACGtt < 1:1422658/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:104418/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:389466/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:634834/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:804507/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:78268/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:602677/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:536535/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:3242406/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:3279948/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:1031323/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:2303440/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:1216517/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:2397895/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:2420747/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:2510832/69‑1 (MQ=255) gCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:2526719/69‑1 (MQ=255) cAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTATTTGGCGTTCGGGTTCAAGAAACCAGAGCGACGtt < 1:1767386/68‑1 (MQ=255) tAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTc > 1:1108060/1‑39 (MQ=255) tAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTc > 1:3320548/1‑39 (MQ=255) tAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGAt > 1:595702/1‑44 (MQ=255) tAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCa > 1:3018547/1‑46 (MQ=255) tAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTca > 1:989566/1‑69 (MQ=255) aaCTCAAAGTAGTTGGTTGTGGTGTATTTGGCGTTCGGTATCAAGAAACCAGAGCGACttt < 1:1115190/61‑1 (MQ=255) cTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTcacccac > 1:29638/1‑69 (MQ=255) cTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTcacccac > 1:532765/1‑69 (MQ=255) cTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTcacccac > 1:1528282/1‑69 (MQ=255) cTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTcacccac > 1:986875/1‑69 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:1604022/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:649578/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:2626637/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:1967200/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:2673706/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:2439173/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:2783271/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:3093598/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:2283941/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:2252995/68‑1 (MQ=255) agtagtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 1:2127890/68‑1 (MQ=255) agtTGGTGGTGGTGTATTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt < 1:854054/51‑1 (MQ=255) agtTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAAc > 1:1850011/1‑68 (MQ=255) tggtggtggTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCa < 1:3262893/69‑1 (MQ=255) tggtggtggTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCa < 1:334180/69‑1 (MQ=255) tggtggtggTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCa < 1:3368284/69‑1 (MQ=255) tggtggtggTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCa < 1:531569/69‑1 (MQ=255) tggtggtggTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCa < 1:792451/69‑1 (MQ=255) tggtggtggTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCa < 1:3092808/69‑1 (MQ=255) tggtggtggTGTACTTGGCGTTCGGGAGCAAGAACCCAGAGCGACGTTTGTCACCCACCGGCAACTGCa < 1:3007227/69‑1 (MQ=255) tggtggtgTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCaaa < 1:1523011/68‑1 (MQ=255) tggtggtgTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCaaa < 1:2427734/68‑1 (MQ=255) tggtggtgTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCaaa < 1:1346620/68‑1 (MQ=255) tggtggtgTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCaaa < 1:44233/68‑1 (MQ=255) | CATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAA > minE/54023‑54143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |