| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 468,888 | C→T | 26.1% | V304V (GTC→GTT) | sucB → | dihydrolipoyltranssuccinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 468,888 | 0 | C | T | 26.1% | 147.9 / 46.1 | 122 | V304V (GTC→GTT) | sucB | dihydrolipoyltranssuccinase |
| Reads supporting (aligned to +/- strand): ref base C (49/41); new base T (11/21); total (60/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
TGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGG > minE/468834‑468956 | tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCTGTTAAAGGCCGTGACg > 1:1000384/1‑68 (MQ=255)tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCTGTTAAAGGCCGTGACg > 1:2465004/1‑68 (MQ=255)tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCTGTTAAAGGCCGTGACg > 1:3002209/1‑68 (MQ=255)tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCTGTTAAAGGCCGTGACg > 1:214759/1‑68 (MQ=255)tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCTGTTAAAGGCCGTGACg > 1:2680755/1‑68 (MQ=255)tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCTGTTAAAGGCCGTGACg > 1:691850/1‑68 (MQ=255)tGTCAATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCTGTTAAAGGCCGTGACg > 1:1808233/1‑68 (MQ=255) tCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGc > 1:907060/1‑68 (MQ=255) aCCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTg > 1:3235860/1‑69 (MQ=255) aCCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTg > 1:2433913/1‑69 (MQ=255) aCCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTg > 1:2385584/1‑69 (MQ=255) aCCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTg > 1:959400/1‑69 (MQ=255) cTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGt > 1:2812673/1‑54 (MQ=255) cTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:2034396/1‑69 (MQ=255) cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGt < 1:263998/69‑1 (MQ=255) cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGt < 1:3409189/69‑1 (MQ=255) cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGt < 1:312063/69‑1 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:7170/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:2719872/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:2360415/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:1960190/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:410009/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:1873606/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:351953/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGCCCGTGACGGCAAGCTGAc > 1:1781561/1‑62 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTTACGGCa > 1:2854849/1‑52 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:2447632/52‑1 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:2772115/52‑1 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa > 1:2882963/1‑52 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa > 1:282122/1‑52 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:2696252/52‑1 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa > 1:2373117/1‑52 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa > 1:2724672/1‑52 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:2334865/52‑1 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:2201701/52‑1 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:2197469/52‑1 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:2029454/52‑1 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:3380813/52‑1 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa > 1:619069/1‑52 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGa > 1:1153880/1‑68 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:601214/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:731965/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:2686793/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:943191/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:3335682/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTCACGGCa < 1:1272039/52‑1 (MQ=255) cAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCa < 1:2643778/51‑1 (MQ=255) aGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCa < 1:1824293/35‑1 (MQ=255) aGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCa < 1:3099029/35‑1 (MQ=255) aGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCa < 1:1095056/35‑1 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAGCGTTGAAGATCTGACCGGTGGTAACTtc > 1:192661/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACtt > 1:3050523/1‑67 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:2243755/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:1471688/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:1510229/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:2870345/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:1727959/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:2441474/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:2251091/1‑68 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAATATCTGACCGGTGGTAACTtcacc < 1:706358/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:845816/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:1034059/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:2577654/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:2555404/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:194396/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:2407891/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:1245343/69‑1 (MQ=255) agagCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGt < 1:1739757/39‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAATCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1856544/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2769601/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2539807/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2525069/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:414539/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:103853/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1859582/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2336190/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:3404601/69‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:593063/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1965936/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1546993/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1351823/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2422217/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:291885/68‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:833679/62‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:1245295/62‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:2740626/62‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:3360326/62‑1 (MQ=255) gCAGGCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:537265/62‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:196037/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:1638593/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:1355750/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:74645/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:3220628/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:2437742/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:1455461/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:3302796/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:2374726/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:2519356/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtg > 1:550432/1‑68 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACAGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:3407585/1‑69 (MQ=255) | TGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGG > minE/468834‑468956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |