| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 468,912 | C→G | 35.3% | T312T (ACC→ACG) | sucB → | dihydrolipoyltranssuccinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 468,912 | 0 | C | G | 35.3% | 117.2 / 108.9 | 132 | T312T (ACC→ACG) | sucB | dihydrolipoyltranssuccinase |
| Reads supporting (aligned to +/- strand): ref base C (37/48); new base G (25/21); total (63/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01 | |||||||||||
Only 100 of 141 total aligned reads displayed.
CTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATG > minE/468844‑468975 | cTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:2034396/1‑69 (MQ=255) cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGt < 1:3409189/69‑1 (MQ=255) cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGt < 1:312063/69‑1 (MQ=255) cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGt < 1:263998/69‑1 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:351953/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:2719872/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:2360415/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:635981/1‑63 (MQ=255) aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGAcc > 1:7170/1‑63 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:943191/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:3366453/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:2686793/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:2212954/1‑69 (MQ=255) gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGAt > 1:1061835/1‑69 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACtt > 1:3050523/1‑67 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:1727959/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:2251091/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:2870345/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:596849/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:3405461/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:721142/1‑68 (MQ=255) tCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc > 1:1471688/1‑68 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:194396/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:2282806/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:1034059/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:2407891/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:2555404/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:2577654/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:845816/69‑1 (MQ=255) aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTCACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:1229416/69‑1 (MQ=255) agagCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGt < 1:1739757/39‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAATCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1856544/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:414539/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2539807/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2769601/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2336190/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:932571/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:3404601/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1859582/69‑1 (MQ=255) cTGGCTGTTAAAGGCCGTGACGGCAAGATGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:2901684/69‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1351823/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1546993/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:291885/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:593063/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:195486/68‑1 (MQ=255) tGGCTGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc < 1:1965936/68‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:1245295/62‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:2740626/62‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:1924962/62‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:3360326/62‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:2475712/62‑1 (MQ=255) gCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccat < 1:2440764/62‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:196037/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:3220628/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:2366500/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:2437742/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:523406/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:74645/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:1804330/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:2530486/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTtc < 1:1355750/52‑1 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:2519356/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:1455461/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:3302796/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:16422/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:2012171/1‑69 (MQ=255) cAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtg > 1:550432/1‑68 (MQ=255) aaaGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtggt > 1:3313947/1‑69 (MQ=255) aaaGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtggt > 1:1602633/1‑69 (MQ=255) aaaGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtgg > 1:3372166/1‑68 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGCCCGGTGGTAACTtcaccat > 1:1422084/1‑48 (MQ=38) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:432671/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:478478/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:541096/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:549581/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:601737/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:691624/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:2556312/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:2102383/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:1034052/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:1154329/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:1796727/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:188382/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:1888427/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:2197994/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:2376408/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:103060/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:2613672/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:2917648/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcaccat > 1:304935/1‑48 (MQ=255) tGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAACTtcacca > 1:779885/1‑47 (MQ=255) ggCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTc < 1:351440/68‑1 (MQ=255) ggCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTc < 1:2056307/68‑1 (MQ=255) ggCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTc < 1:2872781/68‑1 (MQ=255) gCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTc < 1:779924/67‑1 (MQ=255) aGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTg < 1:2657174/68‑1 (MQ=255) gCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:2468627/37‑1 (MQ=255) gCTGACCGTTGAAGATCTGACCGGTGGTAACTtcacc < 1:1071875/37‑1 (MQ=255) gCTGAACGTTGAAGATCTGACCGGTGGTAACTtctcc < 1:862557/37‑1 (MQ=37) cTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATg > 1:3230053/1‑69 (MQ=255) | CTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATG > minE/468844‑468975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |