| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 471,372 | C→T | 56.0% | G246G (GGC→GGT) | sucD → | succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 471,372 | 0 | C | T | 56.0% | 6.3 / 37.8 | 50 | G246G (GGC→GGT) | sucD | succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (14/8); new base T (8/20); total (22/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01 | |||||||||||
AAGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTG > minE/471316‑471427 | aaGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGc < 1:1088038/68‑1 (MQ=255)aaGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGc < 1:2418232/68‑1 (MQ=255)aaGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGc < 1:2069439/68‑1 (MQ=255)aaGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGc < 1:2419921/68‑1 (MQ=255) ccAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCcatc < 1:2645137/69‑1 (MQ=255) gtgGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTTCCATCATTGcc > 1:804620/1‑69 (MQ=255) gtgGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGc > 1:528124/1‑68 (MQ=255) aCATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGa < 1:2957823/69‑1 (MQ=255) aCATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGa < 1:747768/69‑1 (MQ=255) gCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAggg < 1:1935498/69‑1 (MQ=255) tgACTGCGCCGAAAGGCAACCGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:3352873/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:3295216/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:3212442/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1116057/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:2860504/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:2562016/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:2516445/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:406810/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:639342/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:2304636/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:2170954/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1986798/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1170156/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1800720/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1632753/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1410580/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1504709/61‑1 (MQ=255) tgACTGCGCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGACGGTGGTAAAgg < 1:300281/61‑1 (MQ=255) tgACTGCCCCGAAAGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1624947/61‑1 (MQ=255) aCTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCgg > 1:1720646/1‑67 (MQ=255) aCTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGAATGCgg > 1:2989167/1‑67 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCCCGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:1253324/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:2282614/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:3408655/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:3305094/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:1526112/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:3180582/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:3005435/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:928445/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAgg > 1:956448/1‑57 (MQ=255) tGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCAACATTGCCGGTGGGAAAgg > 1:1185660/1‑57 (MQ=255) aaaGGCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAgg < 1:1880251/50‑1 (MQ=255) gCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAGGGACTGCGGATGAGAAATTCgctg > 1:1161576/1‑69 (MQ=255) gCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAGGGACTGCGGATGAGAAATTCgctg > 1:968694/1‑69 (MQ=255) gCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAGGGACTGCGGATGAGAAATTCgctg > 1:1327608/1‑69 (MQ=255) gCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAGGGACTGCGGATGAGAAATTCgctg > 1:372042/1‑69 (MQ=255) gCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAGGGACTGCGGATGAGAAATTCgctg > 1:2989555/1‑69 (MQ=255) gCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAGGGACTGCGGATGAGAAATTCgctg > 1:2754468/1‑69 (MQ=255) gCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAGGGACTGCGGATGAGAAATTCgctg > 1:2305955/1‑69 (MQ=255) gCAAACGTATGGGTCACGCAGGTGCGATCATTGCCGGTGGTAAAGGGACTGCGGATGAGAAATTCgct > 1:2999487/1‑68 (MQ=255) | AAGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTG > minE/471316‑471427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |