| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 57,596 | C→T | 82.3% | E143E (GAG→GAA) | rluA ← | pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 57,596 | 0 | C | T | 82.3% | 38.3 / 2.1 | 28 | E143E (GAG→GAA) | rluA | pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32) |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (8/15); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
CGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTG > minE/57531‑57652 | cGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTcc < 1:737419/69‑1 (MQ=255) cAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTccac < 1:122535/69‑1 (MQ=255) ctGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATATTCcaccac > 1:1637666/2‑69 (MQ=255) ctGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATATTCcaccac > 1:2691874/2‑69 (MQ=255) ctGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATATTCcaccac > 1:2525607/2‑69 (MQ=255) ctGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATATTCcaccac > 1:2311421/2‑69 (MQ=255) ctGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATATTCcaccac > 1:2766412/2‑69 (MQ=255) ctGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATATTCcaccac > 1:116477/2‑69 (MQ=255) ctGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATATTCcaccac > 1:1078174/2‑69 (MQ=255) ctGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATATTCcaccac > 1:1074272/2‑69 (MQ=255) aTCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCCCCACTTCATATTCCGTCTGCGCa > 1:1789054/1‑69 (MQ=255) aTCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCa > 1:2076364/1‑69 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:505752/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:3291445/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:937558/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:3093956/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:2826914/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:2812999/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:1056061/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:2302993/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:2051557/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:1854066/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:1762494/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:1344803/68‑3 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCACAGGTTTAcctg < 1:1381018/68‑3 (MQ=255) accacTCTTGCCGTGTTATCCGCCGCATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:2601480/67‑3 (MQ=255) aTCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTg < 1:3002424/69‑1 (MQ=255) ccgcATATTCCACCACTTCATACTCCGTCTGCGCAGGTTTAcctg < 1:1344346/45‑3 (MQ=38) cTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTaa > 1:2937057/1‑46 (MQ=255) cTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTaa > 1:1323651/1‑46 (MQ=255) cTCAACCACTTCATATTCCGTCTGCGCAGGTTTACCAGTTTCGTaa > 1:2559132/1‑46 (MQ=255) | CGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTG > minE/57531‑57652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |