| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 481,958 | C→T | 40.6% | N117N (AAC→AAT) | tolQ → | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 481,958 | 0 | C | T | 40.6% | 37.5 / 59.3 | 86 | N117N (AAC→AAT) | tolQ | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Reads supporting (aligned to +/- strand): ref base C (30/21); new base T (24/11); total (54/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATA > minE/481905‑482025 | gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGaaaa > 1:1002907/1‑68 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGaaaa > 1:2024900/1‑68 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGaaaa > 1:2500003/1‑68 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1568615/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1021750/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1610555/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1727044/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1924794/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1936277/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1947945/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:2058557/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:2088650/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:227788/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:2890701/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:3177682/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:451862/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:58639/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:696022/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:985731/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:993839/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1432045/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1382968/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:133292/1‑69 (MQ=255)gcgcCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCGATGCGTATTTCCATGAATCGTGAACTTGAAAAt > 1:1229025/1‑69 (MQ=255) gTGGAAGGGGCGTCGCGTGCTATTCGTATCTCCATGAACCGTg < 1:1457135/43‑1 (MQ=255) gTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTg < 1:2738190/43‑1 (MQ=255) gTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTg < 1:495698/43‑1 (MQ=255) gTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTg < 1:705888/43‑1 (MQ=255) gTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTg < 1:1256874/43‑1 (MQ=255) gTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTg < 1:2727339/43‑1 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACg > 1:2855434/1‑49 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCg > 1:2249708/1‑58 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTa > 1:1456251/1‑68 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTAc < 1:2457248/69‑1 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTAc < 1:2900488/69‑1 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTAc > 1:2851703/1‑69 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTAc < 1:858241/69‑1 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTAc < 1:1608991/69‑1 (MQ=255) gcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTAc < 1:590747/69‑1 (MQ=255) tGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt < 1:399559/69‑1 (MQ=255) tGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt < 1:1559270/69‑1 (MQ=255) tGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt < 1:2610797/69‑1 (MQ=255) tGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt < 1:1094342/69‑1 (MQ=255) tGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGt < 1:2090171/69‑1 (MQ=255) aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAAc > 1:3148689/1‑41 (MQ=255) aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACg > 1:523652/1‑42 (MQ=255) aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACg > 1:3152395/1‑42 (MQ=255) aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGc < 1:2524585/69‑1 (MQ=255) aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGc < 1:2068815/69‑1 (MQ=255) aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGc < 1:3389927/69‑1 (MQ=255) cGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCta > 1:845889/1‑67 (MQ=255) cGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTcc > 1:212531/1‑69 (MQ=255) cGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTcc > 1:1890690/1‑69 (MQ=255) cGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTcc > 1:712580/1‑69 (MQ=255) cGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc > 1:795096/1‑68 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:502476/66‑1 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:3192139/66‑1 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:1223947/66‑1 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:1561793/66‑1 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:2629577/66‑1 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:259746/66‑1 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:2573301/66‑1 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:1966781/66‑1 (MQ=255) atttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:1305488/66‑1 (MQ=255) tttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:1703836/66‑1 (MQ=255) tttcCATGAATCGTGAACTTGAAAATCTGGAAACGCACATTCCTTTCCTCGGTACGGTTGGCTCCATc < 1:2086056/66‑1 (MQ=255) tctcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATc > 1:619324/1‑68 (MQ=255) tctcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCa > 1:1540489/1‑69 (MQ=255) tctcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCa > 1:107776/1‑69 (MQ=255) tcCATGAACCGTGAACTTGAAAATCTGGAAACGca < 1:2851436/35‑1 (MQ=255) tcCATGAACCGTGAACTTGAAAATCTGGAAACGca < 1:2303358/35‑1 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGtt > 1:3233938/1‑42 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCgg > 1:3144061/1‑48 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCgg > 1:961864/1‑48 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCt > 1:2504075/1‑59 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc > 1:1208275/1‑60 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc > 1:1956924/1‑60 (MQ=255) cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTc > 1:2546931/1‑60 (MQ=255) tGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:2944583/1‑68 (MQ=255) tGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:287538/1‑68 (MQ=255) tGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:2782687/1‑68 (MQ=255) tGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:2018626/1‑68 (MQ=255) tGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:2045241/1‑68 (MQ=255) tGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:3343250/1‑68 (MQ=255) aaCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCg > 1:3329909/1‑66 (MQ=255) aaCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtat > 1:1781463/1‑69 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:1567651/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:1753870/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:1040857/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:1827201/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:1916029/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:1474188/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:429944/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:362538/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:1397792/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:3190534/68‑1 (MQ=255) ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGtata < 1:2877557/68‑1 (MQ=255) | GCGCCGGAAGCCGTAGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATA > minE/481905‑482025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |