BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	484,794	G→A	62.2%	Q200Q (CAG→CAA)	tolB →	periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	484,794	0	G	A	62.2%	71.6 / 87.9	132	Q200Q (CAG→CAA)	tolB	periplasmic protein
Reads supporting (aligned to +/- strand): ref base G (31/19); new base A (23/59); total (54/78)
Fisher's exact test for biased strand distribution p-value = 2.23e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 132 total aligned reads displayed.

CCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTATGTGACCTTCGAAAGCG  >  minE/484728‑484858

cccGTATGAACTGCGCGTATCTGACTATGACGGTTTCAACCAGTTTGTCGTTCACCGTTCACCGCAGcc                                                                >  1:3281338/1‑69 (MQ=255)

cccGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGcc                                                                >  1:2264935/1‑69 (MQ=255)

cccGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGcc                                                                >  1:2537627/1‑69 (MQ=255)

cccGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGcc                                                                >  1:9817/1‑69 (MQ=255)

cccGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGcc                                                                >  1:662890/1‑69 (MQ=255)

cccGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGcc                                                                >  1:1396259/1‑69 (MQ=255)

cccGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGcc                                                                >  1:132542/1‑69 (MQ=255)

      tGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGAt                                                          <  1:3407833/69‑1 (MQ=255)

             cgcgTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCg                                                   >  1:2937740/1‑69 (MQ=255)

             cgcgTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCg                                                   >  1:2206384/1‑69 (MQ=255)

             cgcgTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCg                                                   >  1:544851/1‑69 (MQ=255)

             cgcgTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCg                                                   >  1:3237102/1‑69 (MQ=255)

             cgcgTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCg                                                   >  1:1104906/1‑69 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCTCAACCGCTGATGTCTCCGGCGTg                                              >  1:2222594/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGt                                               >  1:177590/1‑67 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1660071/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1969745/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1973125/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1843361/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:3223962/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:208488/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:175028/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1213996/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1663905/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1609877/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1577843/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1505775/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:3334460/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:2215013/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:2321283/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:1046645/1‑68 (MQ=255)

                   tCTGACTATGACGGTTACAACCAGTTTCTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTg                                              >  1:923067/1‑68 (MQ=255)

                     tGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGt                                            <  1:1343276/68‑1 (MQ=255)

                     tGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGt                                            <  1:1897489/68‑1 (MQ=255)

                     tGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGt                                            <  1:3182831/68‑1 (MQ=255)

                     tGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGt                                            <  1:781989/68‑1 (MQ=255)

                     tGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGt                                            <  1:1308769/68‑1 (MQ=255)

                         tATGACGGTTAGAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:3134686/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCa                                       >  1:696678/1‑69 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCa                                       >  1:1444114/1‑69 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:2785771/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:2565192/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:3024819/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:2962593/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:3053344/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:2778665/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:625285/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:2282228/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:1016619/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:1080493/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:112488/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:976070/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:879842/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:1449984/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:1709651/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:1787541/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:533367/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:455658/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:2250372/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:429726/68‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:669196/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:1028249/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:585446/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:578667/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:3297653/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:1792289/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:2738204/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:1880147/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:2540212/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:2297439/69‑1 (MQ=255)

                         tATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGATGATGTCTCCGGCGTGGTCACCa                                       <  1:2759240/69‑1 (MQ=255)

                          aTGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:3098959/68‑1 (MQ=255)

                                   aCAACCAGTTTGTCGTTCACCGTTCACCGCAGCCTCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTa                             <  1:1474853/69‑1 (MQ=255)

                                   aCAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTa                             <  1:597901/69‑1 (MQ=255)

                                   aCAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTa                             <  1:47451/69‑1 (MQ=255)

                                     aaCCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTaa                            <  1:2795466/68‑1 (MQ=255)

                                     aaCCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTaa                            <  1:1932814/68‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTTGt                                            <  1:2085753/46‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:2312880/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:1178038/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:1265060/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:971107/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:1332080/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:700482/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:6655/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:2591163/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:2101880/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:2173534/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:2554471/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:2175851/69‑1 (MQ=255)

                                           tttGTCGTTCACCGTTCACCGCAACCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt                     <  1:25062/69‑1 (MQ=255)

                                                 gttcaccgttcaccGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTAtgtg               <  1:1530183/69‑1 (MQ=255)

                                                    caccgttcaccgCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTATGTGAcc            >  1:2815045/1‑69 (MQ=255)

                                                    caccgttcaccgCAACCGCTGATGTCTCCGGCGTGGTCAcc                                        <  1:3041242/41‑1 (MQ=255)

                                                    caccgttcaccgCAACCGCTGATGTCTCCGGCGTGGTCACCa                                       <  1:2073783/42‑1 (MQ=255)

                                                           caccgCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTATGTGACCTTCGaaa     >  1:2595018/1‑69 (MQ=255)

                                                           caccgCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTATGTGACCTTCGaaa     >  1:2682123/1‑69 (MQ=255)

                                                           caccgCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTATGTGACCTTCGaaa     >  1:1283585/1‑69 (MQ=255)

                                                           caccgCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTATGTGACCTTCGaaa     >  1:429425/1‑69 (MQ=255)

                                                           caccgCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTATGTGACCTTCGaaa     >  1:335834/1‑69 (MQ=255)

CCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTTATGTGACCTTCGAAAGCG  >  minE/484728‑484858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`