| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 490,274 | G→C | 34.4% | A218A (GCG→GCC) | pnuC → | predicted nicotinamide mononucleotide transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 490,274 | 0 | G | C | 34.4% | 119.4 / 96.6 | 122 | A218A (GCG→GCC) | pnuC | predicted nicotinamide mononucleotide transporter |
| Reads supporting (aligned to +/- strand): ref base G (47/33); new base C (32/10); total (79/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
GTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCATT > minE/490209‑490338 | gtTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTc < 1:1071464/69‑1 (MQ=255)gtTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTc < 1:2291055/69‑1 (MQ=255)gtTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTc < 1:2535789/69‑1 (MQ=255)gtTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTc < 1:1751052/69‑1 (MQ=255)gtTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTc < 1:3021570/69‑1 (MQ=255) tATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCaa > 1:2226321/1‑69 (MQ=255) tATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCaa > 1:333308/1‑69 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:768461/69‑1 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:1859333/69‑1 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:694601/69‑1 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:208614/69‑1 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:2674035/69‑1 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:2807008/69‑1 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:473029/69‑1 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:391977/69‑1 (MQ=255) tttACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTGGATCa < 1:773545/69‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTTTTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:1647140/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa < 1:1650253/58‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa < 1:2883742/58‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa < 1:822311/58‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa < 1:784550/58‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa < 1:2804352/58‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa < 1:1490199/58‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa < 1:2290317/58‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGa < 1:2661153/58‑1 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCaa > 1:1263322/1‑68 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:2372268/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:914185/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:787689/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:3081044/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:3280047/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:533059/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:646405/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:737217/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:73842/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:1160274/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:1180582/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:1242286/1‑69 (MQ=255) tACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAAc > 1:2198799/1‑69 (MQ=255) ccATGTCTGTGGAGTACATCATCCTGACCTTTATTGCCCT‑AACgg > 1:2365711/1‑45 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:309370/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:1034196/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:909388/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:71183/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:1403342/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:1492771/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:503740/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:161604/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:3274379/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:1654302/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:2928128/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:2323593/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:2085090/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:2503928/1‑59 (MQ=255) ccATGTCTCTGGAGTACATCATCCTGACCTATATTGCCCTCAACGGCAGCCGGATGTgg > 1:1845955/1‑59 (MQ=255) caaTGTCTCTGGAGTACATCATCCTGACCTTTATTGCCCTCAACGGCAGCCGGATGTgg > 1:405576/3‑59 (MQ=255) tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg > 1:2450295/1‑64 (MQ=255) tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg > 1:1343660/1‑64 (MQ=255) tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg > 1:203040/1‑64 (MQ=255) tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg > 1:1462898/1‑64 (MQ=255) tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg > 1:847486/1‑64 (MQ=255) tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg > 1:2139174/1‑64 (MQ=255) tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAgcg > 1:1808636/1‑64 (MQ=255) tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCAca > 1:2974184/1‑67 (MQ=255) tGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGa > 1:2418703/1‑67 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTgg > 1:2377516/1‑43 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGa < 1:2951068/60‑1 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGa < 1:433920/60‑1 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGa < 1:3129913/60‑1 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGa < 1:2502289/60‑1 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGa < 1:127387/60‑1 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGa < 1:2994964/60‑1 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCt > 1:187914/1‑68 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCt > 1:3269722/1‑68 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTc > 1:659951/1‑69 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTc > 1:2697375/1‑69 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTc > 1:731642/1‑69 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTc > 1:1724790/1‑69 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTc > 1:1627542/1‑69 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTc > 1:1706344/1‑69 (MQ=255) catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTc > 1:2524406/1‑69 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:1593483/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:2092952/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:490391/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:2432730/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:508670/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:239664/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:1115917/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:3203061/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:1342965/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:299573/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGCGAAAGAGGTTCAcg > 1:2531981/1‑57 (MQ=255) tATTGCCCTCAACGGCAGCCGGATGTGGAACAACAGCGCACGCGAAAGAGGTTCAc > 1:1668949/1‑56 (MQ=255) tGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCAtt < 1:2225329/68‑1 (MQ=255) tGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCAtt < 1:601314/68‑1 (MQ=255) tGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCAtt < 1:3298856/68‑1 (MQ=255) tGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCAtt < 1:3128319/68‑1 (MQ=255) tGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCAtt < 1:2643555/68‑1 (MQ=255) tGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCAtt < 1:1983408/68‑1 (MQ=255) gcgcTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCAtt < 1:1519918/67‑1 (MQ=255) | GTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCATT > minE/490209‑490338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |