| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 491,553 | A→G | 41.1% | S74P (TCG→CCG) | ybgS ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 491,553 | 0 | A | G | 41.1% | 24.7 / 52.2 | 73 | S74P (TCG→CCG) | ybgS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/31); new base G (12/18); total (24/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
TTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACATTTT > minE/491485‑491621 | tttGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGa > 1:2054328/1‑69 (MQ=255) tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagca > 1:3118543/1‑69 (MQ=255) tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagca > 1:2145434/1‑69 (MQ=255) tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagca > 1:2113499/1‑69 (MQ=255) tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagc > 1:3108860/1‑68 (MQ=255) tGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagc > 1:1658462/1‑68 (MQ=255) ggTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAt < 1:2603478/69‑1 (MQ=255) ggTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAt < 1:734273/69‑1 (MQ=255) ggTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAt < 1:2109600/69‑1 (MQ=255) ggTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAt < 1:762395/69‑1 (MQ=255) gtgtTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAtt > 1:1536689/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGAt > 1:2734975/1‑48 (MQ=39) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTgcc > 1:3260901/1‑61 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:2286076/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:642777/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:446788/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:1942571/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:1686868/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:1913039/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:508155/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:3238984/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:2589075/1‑69 (MQ=255) ctTCATCTTTGGTCATGCCGTCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAAc > 1:1233973/1‑69 (MQ=255) cATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCgg > 1:2531795/1‑69 (MQ=255) cATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCgg > 1:2833525/1‑69 (MQ=255) tCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGt < 1:2905716/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGt < 1:2765522/67‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:2728220/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:2686655/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:1355129/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:71393/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:36467/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:895279/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:2439743/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:2497568/68‑1 (MQ=255) cTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTa < 1:1765737/68‑1 (MQ=255) gTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTAtt < 1:2646603/65‑1 (MQ=255) gTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTAtt < 1:1365132/65‑1 (MQ=255) gTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:967966/68‑1 (MQ=255) ttCCGGCATTGTTCATTGAAGAACCATCCGAATGCAGCAGTGTGCCGCCAGACCCGGt < 1:261442/58‑1 (MQ=255) ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:3142893/62‑1 (MQ=255) ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:3144094/62‑1 (MQ=255) ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:3280036/62‑1 (MQ=255) ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:1011490/62‑1 (MQ=255) ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:2564687/62‑1 (MQ=255) ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:531736/62‑1 (MQ=255) ccGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGTCGCCAGAACCGGTATTTAc < 1:1523151/62‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCATAACCGGTATTTACCCCGt < 1:1680010/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:2988470/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:3315829/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:3301363/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:935190/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:931244/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:1231692/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:1595711/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:737853/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:3090178/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:1775606/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:1840546/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:547563/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:2804408/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:28037/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:524764/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:2708141/64‑1 (MQ=255) tCATTGTTCATTGAAGAACCATCCGGATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGt < 1:2703405/64‑1 (MQ=255) gTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGAc > 1:2573297/1‑69 (MQ=255) tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:3330725/52‑1 (MQ=255) tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:995023/52‑1 (MQ=255) tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:2512587/52‑1 (MQ=255) tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:1270871/52‑1 (MQ=255) tCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTAc < 1:3310366/52‑1 (MQ=255) gaagaaCCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTAtt > 1:2805533/1‑68 (MQ=255) gaagaaCCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTAtt > 1:2410083/1‑68 (MQ=255) cGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAt > 1:763677/1‑68 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:703413/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1031397/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1116987/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1204100/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1205367/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1230036/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1666977/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:1682905/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2133135/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:661910/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2422985/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:54688/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2476519/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2595640/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:2983911/69‑1 (MQ=255) aaTGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACAtttt < 1:3194690/69‑1 (MQ=255) | TTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATTTACCCCGTTATTGTCGACGTTATTTGGCGCGACATTTT > minE/491485‑491621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |