BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	493,466	A→G	22.5%	L195L (CTT→CTC)	gpmA ←	phosphoglyceromutase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	493,466	0	A	G	22.5%	168.5 / 41.3	128	L195L (CTT→CTC)	gpmA	phosphoglyceromutase 1
Reads supporting (aligned to +/- strand): ref base A (55/44); new base G (7/22); total (62/66)
Fisher's exact test for biased strand distribution p-value = 3.19e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGAT  >  minE/493401‑493513

tCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGa                                              <  1:2730258/69‑1 (MQ=255)

tCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGa                                              <  1:1259158/69‑1 (MQ=255)

tCATACACCACCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGa                                              <  1:1440245/69‑1 (MQ=255)

  aTAAACCAGCGGCGCGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata                                            >  1:2220092/1‑69 (MQ=255)

  aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata                                            >  1:3396864/1‑69 (MQ=255)

  aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata                                            >  1:2225988/1‑69 (MQ=255)

  aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata                                            >  1:52256/1‑69 (MQ=255)

  aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata                                            >  1:2918793/1‑69 (MQ=255)

  aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTATTCTTCGCTCATGTTATCGAGata                                            >  1:607464/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCa                                                 >  1:799564/1‑61 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:2815443/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:2861556/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:431609/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:2506868/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:408760/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:3349125/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:604935/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:2078115/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:2138636/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:1845784/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         <  1:1716723/69‑1 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         <  1:784128/69‑1 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:103982/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         <  1:1332250/69‑1 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:1187565/1‑69 (MQ=255)

     cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:1126026/1‑69 (MQ=255)

       ccAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTc                                        <  1:3424528/68‑1 (MQ=255)

       ccAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTc                                        <  1:3122361/68‑1 (MQ=255)

       ccAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTc                                        <  1:105548/68‑1 (MQ=255)

               aCGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat                                           <  1:730734/57‑1 (MQ=255)

               aCGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat                                           >  1:1189579/1‑57 (MQ=255)

               aCGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat                                           >  1:2773346/1‑57 (MQ=255)

               aCGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat                                           >  1:2608027/1‑57 (MQ=255)

               aCGCCAGTCGGGACATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat                                           <  1:218321/57‑1 (MQ=255)

                cGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat                                           <  1:3004080/56‑1 (MQ=255)

                  ccAGTCGGTATATTAAGCTCAAGAATCTATTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg                             >  1:2874851/1‑68 (MQ=255)

                  ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg                             >  1:3388046/1‑68 (MQ=255)

                  ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg                             >  1:286435/1‑68 (MQ=255)

                  ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg                             >  1:1552145/1‑68 (MQ=255)

                  ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg                             >  1:2558449/1‑68 (MQ=255)

                  ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg                             >  1:1271974/1‑68 (MQ=255)

                      tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag                        <  1:1657979/69‑1 (MQ=255)

                      tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag                        <  1:2290050/69‑1 (MQ=255)

                      tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag                        <  1:471626/69‑1 (MQ=255)

                      tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag                        <  1:1841700/69‑1 (MQ=255)

                      tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGAGCACGTAAag                        <  1:2285721/69‑1 (MQ=255)

                              ttAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCg                >  1:872368/1‑69 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTGATGTTATCAAGATATTTCACCAGCGCACGTa                           >  1:274524/1‑56 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:2289981/1‑42 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:1868451/1‑42 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                         >  1:274128/1‑42 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGt                            >  1:2360653/1‑55 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           <  1:1909218/56‑1 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           <  1:1210464/56‑1 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           >  1:1998138/1‑56 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           <  1:3103665/56‑1 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           >  1:13823/1‑56 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           >  1:2465035/1‑56 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           >  1:1781835/1‑56 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           >  1:1781092/1‑56 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa                           >  1:1606149/1‑56 (MQ=255)

                                aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCgtg              >  1:1934677/1‑69 (MQ=255)

                                        gAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGc        <  1:2809498/67‑1 (MQ=255)

                                        gAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGc        <  1:643964/67‑1 (MQ=255)

                                          aTCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag                        <  1:797373/49‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCAGGTAACGAGTTACCGTGTGCGGCgatga   <  1:2326964/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:3190700/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2963255/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:1342709/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:1641599/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:1242029/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2743773/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:126108/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2614553/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2000539/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2397630/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2396325/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:478198/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:1324722/69‑1 (MQ=255)

                                           tctcTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatga   <  1:1780701/69‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:718011/68‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:104578/68‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2274813/68‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2120006/68‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2061143/68‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  <  1:1444079/69‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  <  1:3267254/69‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  >  1:2907499/1‑69 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  <  1:1564634/69‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  <  1:2355062/69‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  <  1:1965788/69‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  <  1:645421/69‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  <  1:1654999/69‑1 (MQ=255)

                                            ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGAgatgat  <  1:5299/69‑1 (MQ=255)

                                                    cGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgat     <  1:408664/58‑1 (MQ=255)

                                                    cGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgat     <  1:1877513/58‑1 (MQ=255)

                                                       tCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga   <  1:2855308/57‑1 (MQ=255)

                                                            ttATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  >  1:1931395/1‑53 (MQ=255)

                                                            ttATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  >  1:3359533/1‑53 (MQ=255)

                                                            ttATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat  >  1:1386858/1‑53 (MQ=255)

TCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGAT  >  minE/493401‑493513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`