| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 58,978 | A→G | 30.5% | A655A (GCT→GCC) | hepA ← | RNA polymerase‑associated helicase protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 58,978 | 0 | A | G | 30.5% | 41.0 / 20.8 | 49 | A655A (GCT→GCC) | hepA | RNA polymerase‑associated helicase protein |
| Reads supporting (aligned to +/- strand): ref base A (14/20); new base G (3/12); total (17/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GCTTCATGTTGCTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGT > minE/58916‑59040 | gcTTCATGTTGCTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGa < 1:1405553/69‑1 (MQ=255)gcTTCATGTTGCTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGa < 1:763282/69‑1 (MQ=255) tCATGTTGCTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCa > 1:2536428/1‑64 (MQ=255) tCATGTTGCTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCa > 1:418591/1‑64 (MQ=255) tCATGTTGCTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCa > 1:498825/1‑64 (MQ=255) tCATGTTGCTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCa > 1:1922102/1‑64 (MQ=255) cTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCa < 1:130144/68‑1 (MQ=255) cAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTg < 1:1123174/69‑1 (MQ=255) cAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTg < 1:3000397/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:1493557/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:1073528/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:511756/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:649676/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:1865162/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:1818819/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:1729635/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:2703613/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:149080/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:1468782/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:1418154/69‑1 (MQ=255) gTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGta < 1:1228756/69‑1 (MQ=255) tttGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTg > 1:1962002/1‑64 (MQ=255) tttGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTg > 1:130940/1‑64 (MQ=255) tttGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATAc > 1:927985/1‑69 (MQ=255) tttGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATAc > 1:3198619/1‑69 (MQ=255) tttGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATAc > 1:2223996/1‑69 (MQ=255) tttGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATAc > 1:2045849/1‑69 (MQ=255) tttGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGACAGATAGTTAATCAGATCGTTg > 1:3246476/1‑64 (MQ=255) aTCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATACGCt < 1:1592710/68‑1 (MQ=255) aTCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATACGCTATCGTa < 1:3124187/69‑1 (MQ=255) cGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATACGCTATCGTaaa < 1:2629632/69‑1 (MQ=255) cAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATa > 1:2702309/1‑68 (MQ=255) cAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATAg > 1:1407218/1‑69 (MQ=255) cAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATAg > 1:1183622/1‑69 (MQ=255) tCGGTCTCATCCGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCg > 1:2336275/1‑69 (MQ=255) tCGGTCTCATCCGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCg > 1:2601154/1‑69 (MQ=255) tCGGTCTCAACCGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCg > 1:1594538/1‑69 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTTTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:318766/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:3110144/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:3149819/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:3194565/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:310784/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:2857940/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:27762/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:229537/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:549355/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:1934483/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:83276/69‑1 (MQ=255) cGGCGAGGCCAGATAGTTAATCAAATCGTTGTACACGCTATCGTAGATAGTGCGTCCGGTCGGGCAGgt < 1:109174/69‑1 (MQ=255) | GCTTCATGTTGCTCGCGGCAGTTTTTGATCAGATCGTCAAAGCCTTCGGTTTGATCCGGTGAAGCCAGATAGTTAATCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGT > minE/58916‑59040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |