| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 498,939 | C→G | 37.5% | L435L (CTG→CTC) | modF ← | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 498,939 | 0 | C | G | 37.5% | 37.7 / 48.1 | 56 | L435L (CTG→CTC) | modF | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (21/14); new base G (8/13); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
CAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAA > minE/498872‑498980 | caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:2238754/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:3282316/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:3315671/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:2792827/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:2748020/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:2646899/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:247304/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:492365/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:123765/1‑69 (MQ=255)caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:1270720/1‑69 (MQ=255) tGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGc > 1:1014085/1‑69 (MQ=255) ttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTg < 1:410880/69‑1 (MQ=255) cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:1292758/68‑1 (MQ=255) cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:2903530/68‑1 (MQ=255) cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:2859579/68‑1 (MQ=255) cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:2733672/68‑1 (MQ=255) cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:2624943/68‑1 (MQ=255) cTTAGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:1943547/68‑1 (MQ=255) cTAATCAGCACATCAAGAAACCGGCGGATAAGCTGTCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1998065/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:123339/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1424623/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:2651521/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:3430382/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1762098/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1610454/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1699083/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:3330898/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:2224863/68‑1 (MQ=255) cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:2104729/68‑1 (MQ=255) aaTCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:629693/66‑1 (MQ=255) aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCa > 1:464077/1‑66 (MQ=255) cAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCgag < 1:2606357/69‑1 (MQ=255) aGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:2517250/62‑1 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGc > 1:2147243/1‑42 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:2921784/1‑69 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:1183175/1‑69 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:1650101/69‑1 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:47104/69‑1 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:2508704/69‑1 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:2502908/69‑1 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:2434371/1‑69 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:24242/69‑1 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:2418309/69‑1 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:2073091/1‑69 (MQ=255) aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:938710/1‑69 (MQ=255) aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:3152721/1‑69 (MQ=255) aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:3055375/1‑69 (MQ=255) aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:2988793/1‑69 (MQ=255) aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:2814663/1‑69 (MQ=255) aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1868272/1‑69 (MQ=255) aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1794305/1‑69 (MQ=255) aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1171867/1‑69 (MQ=255) aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:2799463/1‑69 (MQ=255) aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1934770/1‑69 (MQ=255) aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1882453/1‑69 (MQ=255) aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCa > 1:935521/1‑68 (MQ=255) | CAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAA > minE/498872‑498980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |