| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 499,428 | A→T | 30.7% | D272E (GAT→GAA) | modF ← | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 499,428 | 0 | A | T | 30.7% | 59.9 / 32.0 | 52 | D272E (GAT→GAA) | modF | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (20/16); new base T (5/11); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01 | |||||||||||
TTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACG > minE/499361‑499493 | ttGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGAt < 1:3009081/69‑1 (MQ=255)ttGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGAt < 1:1224635/69‑1 (MQ=255)ttGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGAt < 1:2810520/69‑1 (MQ=255)ttGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGAt < 1:2335415/69‑1 (MQ=255)ttGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGAt < 1:2326481/69‑1 (MQ=255) tGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGAt < 1:2693659/68‑1 (MQ=255) cccGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGt < 1:2931611/68‑1 (MQ=255) cccGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGt < 1:102963/68‑1 (MQ=255) cccGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGt < 1:2216279/68‑1 (MQ=255) cccGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGt < 1:3342208/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:3309338/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:2941201/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:2865919/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:2851888/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:3207691/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:2580133/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:577934/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:1868379/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:1519809/68‑1 (MQ=255) ccAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:584477/68‑1 (MQ=255) ttCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCAtt > 1:1964662/1‑69 (MQ=255) tGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCa < 1:1515219/56‑1 (MQ=255) cACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCACGCCATTGTTCAGCACaa > 1:943160/1‑68 (MQ=255) cACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCACGCCATTGTTCAGCACaa > 1:1139517/1‑68 (MQ=255) cACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCACGCCATTGTTCAGCACAAt > 1:2536582/1‑69 (MQ=255) cACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCACGCCATTGTTCAGCACAAt > 1:127817/1‑69 (MQ=255) cACCTGCCAGCTAAGGTTATTAAGAATGGGGCGTTCGTTATAAGAAACCACGCCATTGTTCAGCACAAt > 1:3129111/1‑69 (MQ=255) attaAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgg > 1:1709456/1‑68 (MQ=255) attaAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgg > 1:719680/1‑68 (MQ=255) attaAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgg > 1:18164/1‑68 (MQ=255) attaAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgg > 1:2267626/1‑68 (MQ=255) attaAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgg > 1:2842060/1‑68 (MQ=255) attaAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgg > 1:941139/1‑68 (MQ=255) attaAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgg > 1:1340481/1‑68 (MQ=255) attaAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCg > 1:2886221/1‑67 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:643890/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:2654820/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:400710/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:2191606/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:2150731/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:1933295/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:1929204/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:1722224/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:1660309/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:1388378/1‑69 (MQ=255) tGGGGCGATCGTTATAAGAAACCACGACATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt > 1:165248/1‑69 (MQ=255) gCGATCGTTATAAGATACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACg < 1:1073557/69‑1 (MQ=255) gCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACg < 1:2992578/69‑1 (MQ=255) gCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACg < 1:3180808/69‑1 (MQ=255) gCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACg < 1:2841230/69‑1 (MQ=255) gCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACg < 1:3432793/69‑1 (MQ=255) gCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACg < 1:675538/69‑1 (MQ=255) | TTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACG > minE/499361‑499493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |