Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 499,568 C→T 5.8% A226T (GCA→ACA)  modF ← fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE499,5680CT 5.8% 375.9 / 4.2 166A226T (GCA→ACA) modFfused subunits of molybdate transporter and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (98/58);  new base T (5/5);  total (103/63)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

TCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAAC  >  minE/499504‑499635
                                                                |                                                                   
tCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCttgt                                                                 <  1:1044139/69‑1 (MQ=255)
tCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCttgt                                                                 <  1:745948/69‑1 (MQ=255)
tCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCttgt                                                                 <  1:1141326/69‑1 (MQ=255)
tCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCttgt                                                                 <  1:3071624/69‑1 (MQ=255)
tCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCttgt                                                                 <  1:1166231/69‑1 (MQ=255)
 cATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCttgt                                                                 <  1:2270783/68‑1 (MQ=255)
               aGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCttgt                                                                 <  1:938272/54‑1 (MQ=255)
                 ttGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCttgt                                                                 <  1:2672199/52‑1 (MQ=255)
                        ccTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCa                                         >  1:2036734/1‑69 (MQ=255)
                        ccTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCa                                         >  1:2753421/1‑69 (MQ=255)
                        ccTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCa                                         >  1:3175068/1‑69 (MQ=255)
                        ccTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCa                                         >  1:1703451/1‑69 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:129180/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:2582413/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:271381/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:2945363/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:1152133/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:3202107/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:32155/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:3240543/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:332043/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:1513830/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:147555/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:458786/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:651688/69‑1 (MQ=255)
                          ttCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTAGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       <  1:2190941/69‑1 (MQ=255)
                                 tGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCt                                >  1:398818/1‑69 (MQ=255)
                                 tGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGGTTCCGCt                                >  1:355009/1‑69 (MQ=255)
                                         aTGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCa                        >  1:2160191/1‑69 (MQ=255)
                                         aTGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCa                        >  1:1343609/1‑69 (MQ=255)
                                         aTGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCa                        >  1:1998724/1‑69 (MQ=255)
                                         aTGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCa                        >  1:671268/1‑69 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGTTTGTTGGAGCAGTTCCTCTTTAGCGCCAGt                                       >  1:1354461/1‑53 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGTTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCaa                       >  1:820978/1‑69 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGTTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCaa                       >  1:500712/1‑69 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTc                                                      <  1:2686978/38‑1 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTc                                                      <  1:2638577/38‑1 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTc                                                      >  1:2665251/1‑38 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTc                                                      <  1:663792/38‑1 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTc                                                      <  1:1819942/38‑1 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTc                                                      <  1:1582548/38‑1 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTa                                               >  1:3008952/1‑45 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACg                            >  1:1804380/1‑64 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGt                           >  1:3039032/1‑65 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGt                           >  1:3134996/1‑65 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGt                           >  1:1767574/1‑65 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGt                           >  1:1694432/1‑65 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGt                           >  1:3352302/1‑65 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGt                           >  1:1924422/1‑65 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGt                           >  1:2234686/1‑65 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGt                           >  1:719871/1‑65 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCaa                       >  1:1488538/1‑69 (MQ=255)
                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCaa                       >  1:2570822/1‑69 (MQ=255)
                                               cAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATcc                    >  1:1429746/1‑67 (MQ=255)
                                               cAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATcc                    >  1:1828934/1‑67 (MQ=255)
                                                aGTTGCGTAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:310656/1‑69 (MQ=255)
                                                aGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:2070595/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:2163445/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:888208/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:769994/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:1069035/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:1387178/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:3249426/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:3421703/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:1449819/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:546660/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:358825/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:2082878/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:2913650/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:283273/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:2384889/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:2789612/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:2402310/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:1991215/1‑69 (MQ=255)
                                                aGTTGCGCAACGAGTGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgcc                 >  1:1893671/1‑69 (MQ=255)
                                                  ttGCGCGACGAGTGTTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccag               <  1:1041395/69‑1 (MQ=255)
                                                  ttGCGCGACGAGTGTTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccag               <  1:1237213/69‑1 (MQ=255)
                                                  ttGCGCGACGAGTGTTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccag               <  1:2308574/69‑1 (MQ=255)
                                                  ttGCGCGACGAGTGTTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccag               <  1:1636625/69‑1 (MQ=255)
                                                  ttGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccag               <  1:373931/69‑1 (MQ=255)
                                                    gcgcGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGc                         <  1:2525256/57‑1 (MQ=255)
                                                    gcgcGACGAGTGCTTGTTGGAGCAGCTCCTCTTTAGCGCCAGTTTCCGCTAACGTGc                         <  1:3146031/57‑1 (MQ=255)
                                                            aGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccagcacgccagca     <  1:1265191/69‑1 (MQ=255)
                                                            aGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccagcacgccagca     <  1:1672380/69‑1 (MQ=255)
                                                            aGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccagcacgccagca     <  1:2443369/69‑1 (MQ=255)
                                                            aGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccagcacgccagca     <  1:2632120/69‑1 (MQ=255)
                                                            aGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCccccagcacgccagca     <  1:3294593/69‑1 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCcgccagcacgccagca     >  1:407879/1‑67 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCaaa   >  1:1452191/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCaaa   >  1:3354967/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCaaa   >  1:1348138/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCaaa   >  1:908156/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGAAATCCGCCAGCACGCCAGCaaa   >  1:518441/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGAAATCCGCCAGCACGCCAGCaaa   >  1:2972784/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGAAATCCGCCAGCACGCCAGCaaa   >  1:1844970/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGAAATCCGCCAGCACGCCAGCaaa   >  1:2919946/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGAAATCCGCCAGCACGCCAGCaaa   >  1:2101945/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCTTCTTTAGCGCCAGTTTCCGCTAACGTGAAATCCGCCAGCACGCCAGCaaa   >  1:2953223/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCaaa   >  1:14577/1‑69 (MQ=255)
                                                              tGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCaaa   >  1:1639173/1‑69 (MQ=255)
                                                                |                                                                   
TCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAAC  >  minE/499504‑499635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: