| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 505,334 | A→C | 45.6% | V98V (GTA→GTC) | ybhE → | 6‑phosphogluconolactonase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 505,334 | 0 | A | C | 45.6% | 3.6 / 117.9 | 101 | V98V (GTA→GTC) | ybhE | 6‑phosphogluconolactonase |
| Reads supporting (aligned to +/- strand): ref base A (24/31); new base C (22/24); total (46/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
TGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTC > minE/505274‑505400 | tGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCtt > 1:1795440/1‑68 (MQ=255)tGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCtt > 1:2131525/1‑68 (MQ=255)tGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCtt > 1:2907867/1‑68 (MQ=255) cTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTAc < 1:3159427/66‑1 (MQ=255) ggTAGTCCGACGCATATTTCCACCGATCAGCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGt < 1:1360305/69‑1 (MQ=255) ggTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGt < 1:1074642/69‑1 (MQ=255) ggTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGt < 1:1510732/69‑1 (MQ=255) ggTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGt < 1:187455/69‑1 (MQ=255) ggTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGt < 1:710899/69‑1 (MQ=255) tttCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAAcgc > 1:1124437/1‑69 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAAGGCGTCt < 1:8665/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1445759/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1015885/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:216966/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:269898/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:2914955/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1761190/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:2121922/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1851561/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:2037403/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:2951627/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1442844/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1434595/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:252740/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1352014/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1289684/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1240898/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1198015/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:1194564/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:2243957/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:2361592/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:2277968/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:3364912/65‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:218688/69‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:2120615/69‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:3374008/69‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:1824967/69‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:2966433/69‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:2989795/69‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:1623449/69‑1 (MQ=255) gATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:1588167/69‑1 (MQ=255) aTCACCAGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCt < 1:3395274/64‑1 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2332272/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2359878/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:58143/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:444701/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:3221978/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:3091998/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:3014041/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2639726/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2675344/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:3008358/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:519910/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2316228/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2292843/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:647069/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2232300/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:1118902/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:168647/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2116627/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:645696/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:2135528/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGcc > 1:1151462/1‑69 (MQ=255) aGGGGCAGTTTGTCTTTGTCGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGc > 1:1431642/1‑68 (MQ=255) gCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGcc < 1:112287/69‑1 (MQ=255) gCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGcc < 1:529287/69‑1 (MQ=255) gCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGcc < 1:2254102/69‑1 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:3173849/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:3267041/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:1204/53‑1 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:319021/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:1792850/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:371279/53‑1 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:1103491/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:1035633/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:1405610/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:3035991/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:50162/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:1798586/53‑1 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:2146123/53‑1 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:2887947/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:2156782/53‑1 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:2622691/53‑1 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:261781/53‑1 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa > 1:2299776/1‑53 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTgg > 1:2285108/1‑69 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTgg > 1:3316145/1‑69 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTgg > 1:3000368/1‑69 (MQ=255) ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTgg > 1:1707190/1‑69 (MQ=255) ttGTCTTTGTAGG‑TCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGaa < 1:2151141/52‑1 (MQ=255) tGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTggg > 1:338973/1‑69 (MQ=255) tGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTggg > 1:3346149/1‑69 (MQ=255) tGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTggg > 1:1113744/1‑69 (MQ=255) tGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTgg > 1:2165861/1‑68 (MQ=255) tAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCgtcgtc < 1:3349309/68‑1 (MQ=255) tAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCgtcgtc < 1:2953379/68‑1 (MQ=255) tAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCgtcgtc < 1:2861654/68‑1 (MQ=255) tAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCgtcgtc < 1:119756/68‑1 (MQ=255) tAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCgtcgtc < 1:83826/68‑1 (MQ=255) tAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCgtcgtc < 1:899177/68‑1 (MQ=255) | TGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTC > minE/505274‑505400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |