| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 515,011 | T→C | 46.5% | I234M (ATA→ATG) | bioA ← | 7,8‑diaminopelargonic acid synthase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 515,011 | 0 | T | C | 46.5% | 8.0 / 87.9 | 101 | I234M (ATA→ATG) | bioA | 7,8‑diaminopelargonic acid synthase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (37/17); new base C (23/24); total (60/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 101 total aligned reads displayed.
TACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACA > minE/514953‑515074 | tACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCgg > 1:759248/1‑68 (MQ=255)tACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGa > 1:1398114/1‑69 (MQ=255)tACGACCAAATCCAGTCGCGATCTCCTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGa > 1:1351579/1‑69 (MQ=255) aCCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCg < 1:2110181/69‑1 (MQ=255) ccAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc < 1:1841002/69‑1 (MQ=255) ccAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc < 1:246625/69‑1 (MQ=255) ccAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc < 1:1528540/69‑1 (MQ=255) ccAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc < 1:223466/69‑1 (MQ=255) ccAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc < 1:2270392/69‑1 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:340960/1‑68 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:321099/1‑68 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:464019/1‑68 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:3109002/1‑68 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:2151541/1‑68 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:1503026/1‑68 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:238303/1‑68 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:1284253/1‑68 (MQ=255) gCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTc > 1:1240026/1‑68 (MQ=255) ctcGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGAt > 1:3073157/1‑68 (MQ=255) ctcGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGAt > 1:1492595/1‑68 (MQ=255) cAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:2990711/68‑1 (MQ=255) cAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:532579/68‑1 (MQ=255) cAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:3373232/68‑1 (MQ=255) cAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg > 1:2746340/1‑68 (MQ=255) aaTCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATgcg < 1:3066735/69‑1 (MQ=255) aaTCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATgcg < 1:73249/69‑1 (MQ=255) aaTCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATgcg < 1:2271072/69‑1 (MQ=255) aaTCAGCAAGATACCTTCCCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATgcg < 1:1693425/69‑1 (MQ=255) tCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCa < 1:540507/69‑1 (MQ=255) tCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCa < 1:1265642/69‑1 (MQ=255) aGCAAGATACCTTCTCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:2568512/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCTCATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:423867/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGAt > 1:1988750/1‑35 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:1816763/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:275438/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:2538435/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:2869675/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:662019/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:655950/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:1453523/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:444350/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:1716300/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAAc > 1:3405948/1‑45 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTa > 1:1572266/1‑59 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATc > 1:1411715/1‑69 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATc > 1:1752439/1‑69 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATc > 1:1112339/1‑69 (MQ=255) aGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATc > 1:2427824/1‑69 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTGCATg < 1:2850598/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:2972391/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:951662/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:835640/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:358144/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:690299/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:669192/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:1073349/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:6232/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:3106753/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:574360/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:1049269/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:2560097/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:2544669/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:1304235/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:1532312/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:2329234/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:1602726/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:1642280/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:1675135/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:2215070/63‑1 (MQ=255) aGCAAGATACCTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATg < 1:1940161/63‑1 (MQ=255) ccTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcg > 1:1809765/1‑69 (MQ=255) ccTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcg > 1:1302068/1‑69 (MQ=255) ccTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcg > 1:1848298/1‑69 (MQ=255) ccTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcg > 1:263116/1‑69 (MQ=255) ccTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcg > 1:2934358/1‑69 (MQ=255) ccTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgc > 1:337761/1‑68 (MQ=255) cTTCGCGATTGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:2565288/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCa > 1:1574731/1‑37 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACAt > 1:3103615/1‑52 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:519712/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:1175884/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:2553433/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:2370950/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:1308995/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:662234/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:1431333/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:1480000/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:2325486/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:3138354/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:2274305/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:2233101/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:1724429/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:37079/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:1793901/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:2144504/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcgc > 1:3170642/1‑69 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcg > 1:1423669/1‑68 (MQ=255) cTTCGCGATCGCACATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTgcg > 1:397877/1‑68 (MQ=255) tCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACa < 1:2141995/69‑1 (MQ=255) tCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACa < 1:1077970/69‑1 (MQ=255) | TACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACA > minE/514953‑515074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |