| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 522,599 | A→C | 45.6% | P168P (CCT→CCG) | ybhK ← | predicted transferase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 522,599 | 0 | A | C | 45.6% | 0.0 / 91.3 | 79 | P168P (CCT→CCG) | ybhK | predicted transferase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (19/24); new base C (10/26); total (29/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01 | |||||||||||
AGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCATG > minE/522533‑522667 | aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:2119934/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:115002/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:1199902/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:2275358/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:361002/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:3405586/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:3308832/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:1414820/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:1610618/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAgg > 1:1607956/1‑69 (MQ=255)aGGCCCAATAATGATGAGATCCGCTACACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCgg > 1:163816/1‑69 (MQ=255) ataatGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAAc < 1:2785996/69‑1 (MQ=255) ataatGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAAc < 1:2224659/69‑1 (MQ=255) ataatGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAAc < 1:2300774/69‑1 (MQ=255) ataatGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAAc < 1:23348/69‑1 (MQ=255) ataatGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAAc < 1:1597833/69‑1 (MQ=255) ataatGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAAc < 1:2343927/69‑1 (MQ=255) ataatGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAAc < 1:348429/69‑1 (MQ=255) aatGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACa > 1:479419/1‑68 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGc > 1:1693886/1‑60 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACaa > 1:3117180/1‑68 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:2401996/1‑69 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:2412350/1‑69 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:1073183/1‑69 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:3430968/1‑69 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:370642/1‑69 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:642318/1‑69 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:1014387/1‑69 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:1226887/1‑69 (MQ=255) atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:152525/1‑69 (MQ=255) atgatgAGATCCGCTTCACTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAAt > 1:3243171/1‑69 (MQ=255) gatgaGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATaa > 1:3040219/1‑69 (MQ=255) gatgaGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATaa > 1:1843811/1‑69 (MQ=255) tGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAg < 1:3289420/69‑1 (MQ=255) tGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAg < 1:2139079/69‑1 (MQ=255) gATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAg < 1:1953880/68‑1 (MQ=255) gATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGt < 1:2289435/69‑1 (MQ=255) gATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGt < 1:2186368/69‑1 (MQ=255) gCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAAc > 1:983560/1‑69 (MQ=255) gCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAAc > 1:1366319/1‑69 (MQ=255) gCGTGAACCGCCTCACGAGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAAc > 1:453105/1‑69 (MQ=255) cTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATAtt < 1:3359802/69‑1 (MQ=255) tCACGCGTTGCGGGTACATTAGGCGTTAACAATTACTCTTGAATCGGCGTAGTTAACTGGTCGATAtt < 1:1308791/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:3070207/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:3108549/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:3077256/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:3096695/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:3333105/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:1264845/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:1852004/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:395060/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:44154/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:1134679/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:808681/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:918797/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:2991561/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:2282553/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:1755787/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:2893745/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:1549913/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:2890173/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:2877514/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:1459213/68‑1 (MQ=255) gcgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGGCGATATTGAcc < 1:1945781/68‑1 (MQ=255) gcgTTGCGGGTACATTCCGCGTTAACAATAACTCTTGAATCGGCGAAGTTAACTGGTCGATATTGAcc < 1:1594955/68‑1 (MQ=255) cgTTGCGGGTACATTCGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGAcc < 1:1322781/67‑1 (MQ=255) ggTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTa < 1:3173346/68‑1 (MQ=255) ggTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTa < 1:2241615/68‑1 (MQ=255) ggTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTa < 1:1284365/68‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGTGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:1768625/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:2812358/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:1268362/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:2640034/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:213541/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:2269459/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:162959/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:2307624/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:694079/69‑1 (MQ=255) cATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACtt < 1:858684/69‑1 (MQ=255) aGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCa > 1:298203/1‑67 (MQ=255) aGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCAt > 1:2906692/1‑68 (MQ=255) aGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCAt > 1:2946268/1‑68 (MQ=255) aGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCATg > 1:3121948/1‑69 (MQ=255) aGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCATg > 1:897275/1‑69 (MQ=255) aGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCATg > 1:2209359/1‑69 (MQ=255) | AGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCATG > minE/522533‑522667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |