| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 523,609 | A→G | 37.0% | K37K (AAA→AAG) | moaA → | molybdopterin biosynthesis protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 523,609 | 0 | A | G | 37.0% | 55.0 / 63.5 | 100 | K37K (AAA→AAG) | moaA | molybdopterin biosynthesis protein A |
| Reads supporting (aligned to +/- strand): ref base A (26/37); new base G (19/18); total (45/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
CTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCG > minE/523543‑523668 | ctTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACaaa > 1:841572/1‑67 (MQ=255)ctTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACaaa > 1:338430/1‑67 (MQ=255) tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCTTc > 1:2043564/1‑69 (MQ=255) tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTc > 1:2879815/1‑69 (MQ=255) tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTc > 1:3248473/1‑69 (MQ=255) tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTc > 1:636461/1‑69 (MQ=255) tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTc > 1:1451989/1‑69 (MQ=255) gATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCa > 1:1861858/1‑68 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:853067/63‑1 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:2242183/63‑1 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:2503464/63‑1 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:2562805/63‑1 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:2663842/63‑1 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:1872618/63‑1 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:795582/63‑1 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:1353801/63‑1 (MQ=255) tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:1346037/63‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCATTaaa < 1:1313295/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:484209/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2324128/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2133298/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2094253/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:752604/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2505961/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:1993288/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2581469/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:1299777/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:276969/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:1308428/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:322542/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:17863/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:60510/69‑1 (MQ=255) gATGTGTGTAACTTTCGTTGCACATACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATata < 1:1719190/69‑3 (MQ=255) gATGTGTGTAACTTTCGTTGAACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:3392887/69‑1 (MQ=255) aTGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:1788939/68‑1 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGc > 1:659130/1‑68 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:3367257/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:2529525/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:3379604/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:643912/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:878097/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:1854174/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:1428249/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:1081209/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:199023/1‑69 (MQ=255) tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt < 1:1069618/69‑1 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAAcc > 1:313445/1‑44 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCg > 1:2216754/1‑45 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCg > 1:3361475/1‑45 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCg > 1:2875379/1‑45 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCg > 1:2382243/1‑45 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCg > 1:2020901/1‑45 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCttt < 1:3323157/69‑1 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCttt < 1:3434051/69‑1 (MQ=255) tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCttt < 1:2311496/69‑1 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTc > 1:732674/1‑68 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:1943368/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:3315451/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:3262387/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2164530/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2888113/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:178419/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2342221/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2785236/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2720558/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2595162/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:161076/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:559583/1‑69 (MQ=255) tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:593194/1‑69 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCTAGCGGCGTCACCAATAAAGGCTTTCtt < 1:1973863/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:3172592/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:1734674/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:1693156/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:2279934/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:3304073/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:66522/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:2741645/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:1990048/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:871775/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:2110516/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:2508044/68‑1 (MQ=255) aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAACAAAGGCTTTCtt < 1:1647970/68‑1 (MQ=255) tgcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:423600/51‑1 (MQ=255) gcctgccGGATGGCTACAAACCTAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:3320237/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:1274132/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:2270900/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:1123397/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:191690/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:1936672/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:2697785/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:2633675/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:260272/61‑1 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:192561/1‑69 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:679959/1‑69 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:2000804/1‑69 (MQ=255) gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:10264/1‑69 (MQ=255) gcctgcaGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:436070/1‑69 (MQ=255) tGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTAcgcg > 1:1123674/1‑69 (MQ=255) tGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTAcgcg > 1:2411911/1‑69 (MQ=255) aaaCCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:1402292/44‑1 (MQ=255) | CTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCG > minE/523543‑523668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |