| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 523,681 | A→T | 41.3% | R61S (AGA→AGT) | moaA → | molybdopterin biosynthesis protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 523,681 | 0 | A | T | 41.3% | 21.2 / 41.9 | 46 | R61S (AGA→AGT) | moaA | molybdopterin biosynthesis protein A |
| Reads supporting (aligned to +/- strand): ref base A (12/15); new base T (9/10); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTTACCG > minE/523627‑523742 | tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGTCACCGaaaa < 1:3179033/69‑1 (MQ=255)tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:2304756/69‑1 (MQ=255)tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:3081576/69‑1 (MQ=255)tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:811758/69‑1 (MQ=255)tAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:3273726/69‑1 (MQ=255) aaaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:2721583/68‑1 (MQ=255) aaaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:2226454/68‑1 (MQ=255) aaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGaaaa < 1:1302126/67‑1 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:948202/1‑68 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:2000120/1‑68 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1922552/1‑68 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:398531/1‑68 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:694937/1‑68 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1638441/1‑68 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1500313/1‑68 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1498949/1‑68 (MQ=255) ggCTTTCTGACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGtc > 1:1408562/1‑68 (MQ=255) gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAATAGTGCGCCTGACaggag > 1:1759962/1‑67 (MQ=255) gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAATAGTGCGCCTGACaggag > 1:1763868/1‑67 (MQ=255) gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAg > 1:2744425/1‑52 (MQ=255) gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACaggag > 1:746323/1‑67 (MQ=255) gATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACaggag > 1:2121168/1‑67 (MQ=255) aaaTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGc > 1:2415635/1‑69 (MQ=255) aTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGTGGAGAGcc < 1:1074566/68‑1 (MQ=255) aTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGcc < 1:2623213/68‑1 (MQ=255) aTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGcc < 1:1990118/68‑1 (MQ=255) aTTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGcc < 1:2244711/68‑1 (MQ=255) cGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:2755459/1‑68 (MQ=255) cGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:1666902/1‑68 (MQ=255) cGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:1676520/1‑68 (MQ=255) cGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:3342189/1‑68 (MQ=255) cGCCGGGTTACGCGAGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:2177634/1‑68 (MQ=255) cGCCGGATTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTc > 1:7102/1‑68 (MQ=255) ccGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCtt < 1:1697714/68‑1 (MQ=255) ccGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGACGTCtt < 1:1748/68‑1 (MQ=255) cGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCtt < 1:2122027/67‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTAcct < 1:47675/69‑2 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:481750/69‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:637208/69‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:686398/69‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:3387233/69‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:1798732/69‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:3198594/69‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:781625/69‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:2936322/69‑1 (MQ=255) tCGCCAGTCTGGGCACCGAAAAAGTCCGTCTGACAGGTGGTGAGCCATCTTTACGCCGCGACTTTACCg < 1:1047594/69‑1 (MQ=255) | TAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTTACCG > minE/523627‑523742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |