| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 528,351 | T→C | 18.6% | S201S (TCA→TCG) | ybhN ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 528,351 | 0 | T | C | 18.6% | 119.5 / 17.3 | 80 | S201S (TCA→TCG) | ybhN | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (33/32); new base C (7/8); total (40/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTACCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGAAAGCGCAAAAC > minE/528298‑528414 | aTTACCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGc < 1:2592601/69‑1 (MQ=255) aCCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCttt > 1:74162/1‑69 (MQ=255) aCCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCttt > 1:1298297/1‑69 (MQ=255) aCCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCttt > 1:133982/1‑69 (MQ=255) aCCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCttt > 1:2932365/1‑69 (MQ=255) aCCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCttt > 1:2345295/1‑69 (MQ=255) aCCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCttt > 1:200096/1‑69 (MQ=255) aCCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCtt > 1:2998600/1‑68 (MQ=255) tCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGcc < 1:852288/69‑1 (MQ=255) tCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGcc < 1:3264466/69‑1 (MQ=255) tCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGcc < 1:3024811/69‑1 (MQ=255) tCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGcc < 1:3011210/69‑1 (MQ=255) ccAGTTAACGCTGGAAATCAGCATCTGAGCTAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGccc > 1:21142/1‑69 (MQ=255) ccAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGGATTTCCATGAAGGCAGCACCAGCTTTTGccc > 1:2500410/1‑69 (MQ=255) ccAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGgcag > 1:3051920/1‑54 (MQ=255) ccAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCCCCAGCTTTTGccc > 1:839411/1‑69 (MQ=255) ccAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGccc > 1:471772/1‑69 (MQ=255) cAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCt > 1:1225795/1‑69 (MQ=255) cAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCt > 1:2504188/1‑69 (MQ=255) cAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCt > 1:672330/1‑69 (MQ=255) ttAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGGCCTTTg > 1:710281/1‑69 (MQ=255) ttAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTg > 1:658619/1‑69 (MQ=255) ttAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTg > 1:1487979/1‑69 (MQ=255) ttAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTg > 1:1486136/1‑69 (MQ=255) ttAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTg > 1:1894406/1‑69 (MQ=255) ttAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTg > 1:1284477/1‑69 (MQ=255) ttAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTg > 1:1630122/1‑69 (MQ=255) ttAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTg > 1:303552/1‑69 (MQ=255) aaCGCTGGAAATCAGCCTCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCTGCTTTTGCCCTTTGa > 1:61322/1‑68 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCCTGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:256673/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGTTGGTCa < 1:438661/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2849918/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2781327/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2402503/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2269022/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:1547189/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:3069460/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2224890/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2186541/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:682021/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2076031/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:1956723/69‑1 (MQ=255) tGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:172897/69‑1 (MQ=255) gAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:3203217/67‑1 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:1344906/62‑1 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:3353835/62‑1 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2933874/62‑1 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2621032/62‑1 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:453125/62‑1 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:243025/62‑1 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:3341414/62‑1 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCg > 1:3234268/1‑69 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCg > 1:1146922/1‑69 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCg > 1:13041/1‑69 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCg > 1:2926078/1‑69 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCg > 1:2313683/1‑69 (MQ=255) cAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCg > 1:1826368/1‑69 (MQ=255) tGAGCGATGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:516255/55‑1 (MQ=255) tGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:524301/55‑1 (MQ=255) tGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:698626/55‑1 (MQ=255) tGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:2843473/55‑1 (MQ=255) gAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCa < 1:1063043/54‑1 (MQ=255) tgAGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTc < 1:2515654/64‑1 (MQ=255) tgAGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTc < 1:2498643/64‑1 (MQ=255) tgAGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTc < 1:498412/64‑1 (MQ=255) tgAGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTc < 1:2463527/64‑1 (MQ=255) tgAGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTc < 1:2459631/64‑1 (MQ=255) tgAGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTc < 1:1889508/64‑1 (MQ=255) tgAGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTc < 1:1919953/64‑1 (MQ=255) tgAGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTc < 1:2246798/64‑1 (MQ=255) aGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGaaa > 1:1636798/1‑69 (MQ=255) aGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGaaa > 1:796825/1‑69 (MQ=255) aGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGaaa > 1:1820253/1‑69 (MQ=255) aGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGaaa > 1:1575856/1‑69 (MQ=255) aGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGaaa > 1:2326568/1‑69 (MQ=255) aGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGaaa > 1:2374790/1‑69 (MQ=255) aGGGCGAATTTCCACGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGaa > 1:1227580/1‑68 (MQ=255) tCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGAAAGCGCAAAAc > 1:521836/1‑68 (MQ=255) tCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGAAAGCGCAAAAc > 1:1763535/1‑68 (MQ=255) tCCATGAAGGAAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGAAAGCGCAAAAc > 1:1646224/1‑68 (MQ=255) | ATTACCATCCAGTTAACGCTGGAAATCAGCATCTGAGCGAGGGCGAATTTCCATGAAGGCAGCACCAGCTTTTGCCCTTTGATGGTCATATGGCGGTGCTTCGCGAAAGCGCAAAAC > minE/528298‑528414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |