| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 531,429 | A→G | 24.1% | V115V (GTA→GTG) | ybhQ → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 531,429 | 0 | A | G | 24.1% | 36.7 / 7.6 | 33 | V115V (GTA→GTG) | ybhQ | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (14/11); new base G (5/3); total (19/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACC > minE/531373‑531497 | gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTGGTGTCTTTGCTg > 1:1028949/1‑69 (MQ=255)gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTGGTGTCTTTGCTg > 1:974048/1‑69 (MQ=255)gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTGGTGTCTTTGCTg > 1:1581355/1‑69 (MQ=255)gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTGGTGTCTTTGCTg > 1:2454876/1‑69 (MQ=255)gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTGGTGTCTTTGCTg > 1:2264992/1‑69 (MQ=255)gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTg > 1:2630725/1‑69 (MQ=255)gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTg > 1:2626601/1‑69 (MQ=255)gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTg > 1:3392786/1‑69 (MQ=255)gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTg > 1:2319979/1‑69 (MQ=255) gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCt < 1:2744773/68‑1 (MQ=255) gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCt < 1:865814/68‑1 (MQ=255) gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCt < 1:599348/68‑1 (MQ=255) gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCt < 1:2114870/68‑1 (MQ=255) cTGGCAATCCTTGCCGGCCCGGCGGTGGTGTCTTTGCTGGCGa < 1:2922943/43‑1 (MQ=255) cTGGCAATCCTTGCCGGCCCGGCGGTGGTGTCTTTGCTGGCGa < 1:2122655/43‑1 (MQ=255) cTGGCAATCCTTGCCGGCCCGGCGGTGGTGTCTTTGCTGGCGa < 1:1759278/43‑1 (MQ=255) aaTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTa < 1:2100855/68‑1 (MQ=255) aaTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTa < 1:1833923/68‑1 (MQ=255) aTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTaaa < 1:3231876/69‑1 (MQ=255) aTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTaaa < 1:2647801/69‑1 (MQ=255) aTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTaaa < 1:1546683/69‑1 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTaaa > 1:2087253/1‑67 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACa > 1:541957/1‑69 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACa > 1:3408632/1‑69 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACa > 1:955852/1‑69 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACa > 1:309304/1‑69 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACa > 1:1226619/1‑69 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACa > 1:2447066/1‑69 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACa > 1:1692917/1‑69 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACa > 1:1544478/1‑69 (MQ=255) ccTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCAAAACa > 1:2921575/1‑69 (MQ=255) cggcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTAcgcc < 1:2636438/69‑1 (MQ=255) tAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTaaa < 1:592821/50‑1 (MQ=255) aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGAcc > 1:2590629/1‑69 (MQ=255) aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGAcc > 1:473159/1‑69 (MQ=255) aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGAcc > 1:2339506/1‑69 (MQ=255) aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGAcc > 1:1739442/1‑69 (MQ=255) aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGAc > 1:8621/1‑68 (MQ=255) aGTGTCTTTGCAGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGTGTACGCCGCTGAcc > 1:1642361/1‑69 (MQ=255) | GAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACC > minE/531373‑531497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |