| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 534,192 | A→G | 51.3% | G415G (GGT→GGC) | ybhF ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 534,192 | 0 | A | G | 51.3% | 0.3 / 72.3 | 80 | G415G (GGT→GGC) | ybhF | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (16/23); new base G (29/12); total (45/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAG > minE/534128‑534260 | gcgcCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCg < 1:2571085/68‑1 (MQ=255) cgcCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCg > 1:1032716/1‑67 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGTGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:1868100/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:1073692/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:911235/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:723565/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:588648/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:3192614/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:3025563/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:2981765/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:2929101/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:2018360/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:1406937/67‑1 (MQ=255) cccGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTa < 1:1316844/67‑1 (MQ=255) ccATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTgc > 1:2677336/1‑69 (MQ=255) ccATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTgc > 1:2498919/1‑69 (MQ=255) ccATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTgc > 1:2143245/1‑69 (MQ=255) ccATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTgc > 1:3090988/1‑69 (MQ=255) ccATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTgc > 1:309795/1‑69 (MQ=255) ccATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTgc > 1:1922549/1‑69 (MQ=255) ccATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTgc > 1:1958226/1‑69 (MQ=255) cacacCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCa > 1:2432697/1‑68 (MQ=255) cacacCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCa > 1:1184381/1‑68 (MQ=255) agagAAAAAGCGTAAATTCTGTTCGACCGTCCGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGcc < 1:876962/69‑1 (MQ=255) agagAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGcc < 1:748063/69‑1 (MQ=255) gagaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGcc < 1:555376/68‑1 (MQ=255) gagaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGcc < 1:3283215/68‑1 (MQ=255) agaAAAAGCGTAAATTCTGTTCTACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2130328/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2339638/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1143920/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2715885/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1423905/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2632049/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:257398/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1236834/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:692357/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:759308/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1463877/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:246635/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:3361580/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1221088/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1934747/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2934359/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2969123/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:3340730/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:3035041/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1710011/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2146451/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1737741/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:3337634/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2054596/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1892002/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:1905322/1‑57 (MQ=255) agaAAAAGCGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgc > 1:1146711/1‑56 (MQ=255) agaAAAAGCGTAAATTATGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:2201899/1‑57 (MQ=255) agaAAAAGAGTAAATTCTGTTCGACCGTCAGGTTGCCGTAGAGCGAAAATTTTTgcg > 1:199671/1‑57 (MQ=255) cGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCgg < 1:2793860/69‑1 (MQ=255) cGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCgg < 1:1646456/69‑1 (MQ=255) cGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCgg < 1:1808596/69‑1 (MQ=255) cGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCgg < 1:2958087/69‑1 (MQ=255) cGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCgg < 1:878618/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGGCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:2820723/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGATATGCTGGCGCGCTTTACCGGAActc < 1:118675/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:2217191/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:224138/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:1301606/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:1147185/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:2259108/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:3334642/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:2344216/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:2521825/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:1632847/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:518376/69‑1 (MQ=255) gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActc < 1:133726/69‑1 (MQ=255) ttACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActctct > 1:1321178/1‑66 (MQ=255) ttACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActctct > 1:2779417/1‑66 (MQ=255) ttACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActctct > 1:2520428/1‑66 (MQ=255) ttACCGTAGAGCGAAAATTTTT‑‑GCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAActctct > 1:2850313/1‑64 (MQ=255) tACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGAt > 1:2185845/1‑39 (MQ=255) tACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGAt > 1:908630/1‑39 (MQ=255) aCCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAg < 1:2776532/69‑1 (MQ=255) aCCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAg < 1:2089268/69‑1 (MQ=255) aCCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAg < 1:111641/69‑1 (MQ=255) | GCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAG > minE/534128‑534260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |