| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 535,699 | C→T | 57.8% | V243V (GTG→GTA) | ybhG ← | predicted membrane fusion protein (MFP) component of efflux pump, membrane anchor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 535,699 | 0 | C | T | 57.8% | 27.1 / 67.9 | 90 | V243V (GTG→GTA) | ybhG | predicted membrane fusion protein (MFP) component of efflux pump, membrane anchor |
| Reads supporting (aligned to +/- strand): ref base C (18/20); new base T (33/19); total (51/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCTTCATTGAGGACCGTGCC > minE/535639‑535761 | aaGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCacac > 1:830847/1‑69 (MQ=255)aaGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCacac > 1:3073197/1‑69 (MQ=255)aaGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCacac > 1:1979443/1‑69 (MQ=255)aaGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCacac > 1:1942170/1‑69 (MQ=255)aaGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCacac > 1:3265388/1‑69 (MQ=255)aaGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCacac > 1:1487135/1‑69 (MQ=255) tGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTa < 1:2463081/69‑1 (MQ=255) tGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTa < 1:843498/69‑1 (MQ=255) gCCTGGTCAAGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTaaat > 1:3136818/1‑68 (MQ=255) gCCTGGTCAAGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTaaat > 1:2474782/1‑68 (MQ=255) gCCTGGTCAAGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTaaat > 1:2239808/1‑68 (MQ=255) gCCTGGTCAAGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTaaat > 1:198382/1‑68 (MQ=255) gCCTGGTCAAGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTaaat > 1:1724251/1‑68 (MQ=255) gCCTGGTCAAGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTaaat > 1:513077/1‑68 (MQ=255) gCCTGGTCAAGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTaaat > 1:1267392/1‑68 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGt < 1:2956993/67‑1 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGt < 1:2300094/67‑1 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGt < 1:630874/67‑1 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:1034519/1‑69 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:2636352/1‑69 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:2778584/1‑69 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:2921476/1‑69 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:723628/1‑69 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:3221185/1‑69 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:1071921/1‑69 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:530653/1‑69 (MQ=255) cAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGc > 1:2425329/1‑69 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:2533685/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:252787/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:253901/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:2932359/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:264134/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:852619/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:2716487/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:2951092/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:772142/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:3100645/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:961307/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:566907/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:396587/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:408536/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:2400584/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:2034671/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:1973743/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:1681030/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:2231231/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:1532462/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:1416148/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:1269810/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:1182726/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:2297517/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:1113860/1‑64 (MQ=255) aaGATTACGTTCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATAcc > 1:1094575/1‑64 (MQ=255) aaGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGt < 1:548247/66‑1 (MQ=255) aaGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGcc > 1:2843223/1‑69 (MQ=255) aaGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGcc > 1:2126175/1‑69 (MQ=255) aGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGcc < 1:2859484/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:634425/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:670654/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:563701/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:1216151/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:358855/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:1946655/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:2369706/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:2137450/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:2563064/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:2576091/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:2686523/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:2615391/68‑1 (MQ=255) gATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:2254030/68‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:1984550/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:1005699/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:2349361/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:2534238/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:2546187/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:1193866/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:651253/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:1218612/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:2621610/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:267141/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:1483582/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:2810266/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:3427705/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:3334651/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:1714724/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:1757921/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:290946/69‑1 (MQ=255) ttCATCAACATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCACCTTCAtt < 1:2074868/69‑1 (MQ=255) ttCATCAAAATAGGCGCGTACCCACACCGGACGCGTCAGCGAGACGGTAAATACCGTGCCTCCTTCAtt < 1:535001/69‑1 (MQ=255) aGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCTTCATTGAGGACCGTGcc > 1:2151191/1‑69 (MQ=255) cgcACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCa < 1:2291881/45‑1 (MQ=255) | AAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATAAGCGCGCACCCACACCGGACGCGTTAGTGAAACGGTAAACACCGTGCCACCTTCATTGAGGACCGTGCC > minE/535639‑535761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |