| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 540,292 | C→T | 34.6% | L241L (CTG→CTA) | rhtA ← | threonine and homoserine efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 540,292 | 0 | C | T | 34.6% | 55.4 / 40.9 | 75 | L241L (CTG→CTA) | rhtA | threonine and homoserine efflux system |
| Reads supporting (aligned to +/- strand): ref base C (28/21); new base T (7/19); total (35/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAG > minE/540227‑540354 | ggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAg < 1:3115819/68‑1 (MQ=255)ggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAg < 1:221492/68‑1 (MQ=255)ggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAg < 1:1870139/68‑1 (MQ=255)ggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAg < 1:2949992/68‑1 (MQ=255)ggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAg < 1:3144541/68‑1 (MQ=255)ggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAg < 1:37798/68‑1 (MQ=255)ggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAg < 1:1164190/68‑1 (MQ=255) tttCTCCGAGTAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCACCGTACCAAATg > 1:50301/1‑69 (MQ=255) tttCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATg > 1:1883461/1‑69 (MQ=255) tttCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATg > 1:897527/1‑69 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCa < 1:3212818/63‑1 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCa < 1:1538746/63‑1 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCa < 1:196703/63‑1 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2319644/1‑69 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:3183349/1‑69 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2459946/1‑69 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:1624279/1‑69 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2506693/1‑69 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2939354/1‑69 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:181514/1‑69 (MQ=255) tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2201763/1‑69 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:2233307/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:2612658/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:494685/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:2789573/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:1170412/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:1667450/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:1346343/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:628860/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:1291620/66‑1 (MQ=255) gCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATc > 1:2566441/1‑69 (MQ=255) gCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATc > 1:3149018/1‑69 (MQ=255) gccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCAtt < 1:2250387/69‑1 (MQ=255) gccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCAtt < 1:1068348/69‑1 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAACCGGGTGAGGGCAATCAtt > 1:2146239/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGGAATCAtt > 1:962609/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:2455056/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:1234603/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:1994301/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:1334082/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:1482051/1‑69 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:2072244/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1213776/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:751314/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:654301/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1287264/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:391883/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1426988/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:30845/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:295168/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:211344/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1987834/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1885912/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:882021/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:2449199/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:57135/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:3312824/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:379833/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1977010/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGGGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1400508/67‑1 (MQ=255) tCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATaa > 1:108683/1‑69 (MQ=255) tCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATaa > 1:2063448/1‑69 (MQ=255) tCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATaa > 1:2027307/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGTGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:718269/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:430828/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:3398254/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:124891/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:3289984/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:2802642/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:1798366/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:1619604/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:957155/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:1775655/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCa > 1:1718663/1‑68 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTACATCGAATAAGGCag > 1:3110876/1‑69 (MQ=255) | GGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAG > minE/540227‑540354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |