| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 540,307 | C→G | 45.4% | R236R (CGG→CGC) | rhtA ← | threonine and homoserine efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 540,307 | 0 | C | G | 45.4% | 24.1 / 110.1 | 97 | R236R (CGG→CGC) | rhtA | threonine and homoserine efflux system |
| Reads supporting (aligned to +/- strand): ref base C (39/14); new base G (25/19); total (64/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 105 total aligned reads displayed.
TCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGC > minE/540239‑540372 | tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:3183349/1‑69 (MQ=255)tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2459946/1‑69 (MQ=255)tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2506693/1‑69 (MQ=255)tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2319644/1‑69 (MQ=255)tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2201763/1‑69 (MQ=255)tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:2939354/1‑69 (MQ=255)tctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTc > 1:181514/1‑69 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:1170412/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:1291620/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:1667450/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:1346343/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:2612658/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:2789573/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:628860/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:494685/66‑1 (MQ=255) aGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgt < 1:2233307/66‑1 (MQ=255) gCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATc > 1:3149018/1‑69 (MQ=255) gCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATc > 1:2566441/1‑69 (MQ=255) gccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCAtt < 1:1068348/69‑1 (MQ=255) gccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCAtt < 1:2250387/69‑1 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAACCGGGTGAGGGCAATCAtt > 1:2146239/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:1482051/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:1334082/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:2455056/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:1994301/1‑69 (MQ=255) gccagcGCAGGTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCAtt > 1:1234603/1‑69 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:211344/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:2072244/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1987834/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:30845/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1213776/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:295168/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:751314/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1287264/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:654301/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1426988/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:391883/67‑1 (MQ=255) caggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1885912/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:57135/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:2449199/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1977010/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:882021/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:379833/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:3312824/67‑1 (MQ=255) aggTTCCATGCTCATTAGCGTACCAAATGTGCGGGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCg < 1:1400508/67‑1 (MQ=255) tCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATaa > 1:2027307/1‑69 (MQ=255) tCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATaa > 1:108683/1‑69 (MQ=255) tCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATaa > 1:2063448/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGTGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:718269/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:3289984/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:2802642/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:430828/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:957155/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:3398254/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:124891/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:1798366/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:1775655/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCag > 1:1619604/1‑69 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCa > 1:1718663/1‑68 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTACATCGAATAAGGCag > 1:3110876/1‑69 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGTTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg > 1:3232172/2‑67 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGGAGAGCgg > 1:1456927/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:492134/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:1639178/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:429614/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:1686440/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:1506725/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:3420905/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:1290482/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:3160278/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:1808574/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:1007033/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:2528592/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:2893479/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:2834536/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCgg > 1:2817443/2‑68 (MQ=255) taGCGTACCAAATGTGCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGAgg > 1:2977861/2‑68 (MQ=255) aGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTTCAGCGAATAAGGCAGAGCgg > 1:2206709/1‑67 (MQ=255) aGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCa > 1:521656/1‑61 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:1840893/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:3159727/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:1414518/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:63605/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:1734252/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:2348202/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:2846025/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:2800753/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGCCAGAGCGGTCgagag > 1:3151475/1‑68 (MQ=255) ccAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATATCCAGCGAATAAGGCAGAGCGGTCgagag > 1:2217681/1‑68 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGGAATCATTTCCAGCGAATAAGGCAGAGCg > 1:3403174/1‑55 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg > 1:2628623/1‑55 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg < 1:2396636/55‑1 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg > 1:1942440/1‑55 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg > 1:1903985/1‑55 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg > 1:114249/1‑55 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg > 1:907758/1‑55 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg > 1:923800/1‑55 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg < 1:2409625/55‑1 (MQ=255) tGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGc > 1:2539127/1‑69 (MQ=255) tGTCCGTGTTGG‑AAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCg > 1:1766181/1‑54 (MQ=255) | TCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGC > minE/540239‑540372 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |