| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 542,507 | G→C | 46.3% | T336T (ACC→ACG) | ybiP ← | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 542,507 | 0 | G | C | 46.3% | 14.2 / 117.3 | 102 | T336T (ACC→ACG) | ybiP | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base G (21/33); new base C (24/23); total (46/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
CAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTCGTTTGGC > minE/542441‑542569 | cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:1023354/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:775978/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:771995/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:405397/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:3197980/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt > 1:2623036/1‑69 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt > 1:2613256/1‑69 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:2542717/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:2496382/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:2346461/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:1906290/69‑1 (MQ=255)cAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt > 1:1379703/1‑69 (MQ=255)cAGCTGAGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:954940/69‑1 (MQ=255) gTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTa < 1:1913013/68‑1 (MQ=255) gTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTa < 1:179349/68‑1 (MQ=255) aGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGgt < 1:44888/55‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:2152346/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:2156958/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:2283698/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:119707/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:2672813/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:2943623/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:3180216/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:3246095/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:3270569/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:41211/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:530590/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:533232/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:1742652/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:1668786/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:1521260/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:1517374/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:598144/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:1502709/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:730130/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:825827/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:1406446/66‑1 (MQ=255) gTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:874806/66‑1 (MQ=255) tGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTGc < 1:1383312/65‑1 (MQ=255) ctctTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCaaa > 1:376164/1‑69 (MQ=255) ctctTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCaaa > 1:2948783/1‑69 (MQ=255) cAGCACTTTAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2014161/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2035104/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:1259671/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:3316687/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:3393774/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:378952/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:1804237/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:508467/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2677195/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2618409/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:1498400/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:1471599/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2058550/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2254172/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:226238/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:1392427/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2302344/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2319503/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:1244890/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2371439/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:121460/1‑53 (MQ=255) cAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTAGTGTTTTTATCTg > 1:1771155/1‑53 (MQ=255) aaGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTCGTGTTTTTATCTg > 1:2850856/2‑53 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGttttt > 1:2729701/1‑41 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGttttt > 1:183200/1‑41 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGttttt > 1:66900/1‑41 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGttttt > 1:1921618/1‑41 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:1006842/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:547419/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:3008343/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:1290838/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:3298297/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:797729/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:203839/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:2080287/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:2054103/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:2977268/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:2580460/69‑1 (MQ=255) tGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAgg < 1:2679558/69‑1 (MQ=255) cGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAg < 1:1197727/68‑1 (MQ=255) cATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTacac < 1:1785721/69‑1 (MQ=255) tCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCa > 1:96446/1‑69 (MQ=255) tCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCa > 1:639173/1‑69 (MQ=255) tCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCa > 1:1504091/1‑69 (MQ=255) tCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCa > 1:1195294/1‑69 (MQ=255) tCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCa > 1:2704484/1‑69 (MQ=255) tCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCa > 1:2915646/1‑69 (MQ=255) tCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCa > 1:1204979/1‑69 (MQ=255) tCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCa > 1:2328255/1‑69 (MQ=255) aaCGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCt > 1:1045546/1‑69 (MQ=255) aCGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTc > 1:538584/1‑69 (MQ=255) aCGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTc > 1:453359/1‑69 (MQ=255) aCGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACATTCTTTCAGGAAGTACACTTCATCTGCTc > 1:372002/1‑69 (MQ=255) gCTTCTTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTCg < 1:3004541/68‑1 (MQ=255) gCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTCg < 1:2891238/68‑1 (MQ=255) gCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTCg < 1:1162365/68‑1 (MQ=255) tCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTCGTTTGGc < 1:2936249/68‑1 (MQ=255) tCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTCGTTTGGc < 1:2005178/68‑1 (MQ=255) tttGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTCGTTTGGc < 1:2290369/66‑1 (MQ=255) | CAGCTGCGGTTGCGAGTGCTCTTGCGCCAGCACTTGAGCGGTCATATCCAGTAACGCTTCGTCTTTGGTGTTTTTATCTGCTTCAAAATTACCTTCTTTCAGGAAGTACACTTCATCTGCTCGTTTGGC > minE/542441‑542569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |