| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 544,210 | T→G | 34.4% | L37L (CTT→CTG) | mntR → | transcriptional regulator of mntH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 544,210 | 0 | T | G | 34.4% | 67.9 / 71.5 | 90 | L37L (CTT→CTG) | mntR | transcriptional regulator of mntH |
| Reads supporting (aligned to +/- strand): ref base T (32/27); new base G (15/16); total (47/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTCAGGTGGACAT > minE/544143‑544278 | aGTTAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTC‑‑ATAGGCGCATCGGCGCGAGCTGAtt < 1:3074680/69‑1 (MQ=255) ttATTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:993566/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:98567/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:907098/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:2232391/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:1211490/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:2666647/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:284351/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:1890349/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:28851/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:2957650/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:425666/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:369256/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:3205121/69‑1 (MQ=255) ttAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:3220132/69‑1 (MQ=255) tAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGAtt < 1:2222134/68‑1 (MQ=255) aGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTAt > 1:268066/1‑66 (MQ=255) aGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATtg > 1:2041597/1‑68 (MQ=255) aGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATtg > 1:879735/1‑68 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTgatgat < 1:1653360/69‑1 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:3279900/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:377241/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:425877/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:66116/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:763886/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:770215/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:2710277/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:774781/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:102717/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:2173232/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:2066951/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:1415921/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:1434993/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:1471148/1‑69 (MQ=255) aaCGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTGATTgatgat > 1:1732005/1‑69 (MQ=255) aaCATGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTgatgat < 1:2213322/69‑1 (MQ=255) aaCATGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTgatgat < 1:964970/69‑1 (MQ=255) aaCATGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTgatgat < 1:788096/69‑1 (MQ=255) aaCATGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTgatgat < 1:691638/69‑1 (MQ=255) aaCATGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTgatgat < 1:1639921/69‑1 (MQ=255) tGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACg < 1:108998/69‑1 (MQ=255) tGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACg < 1:3397307/69‑1 (MQ=255) tGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACg < 1:3263656/69‑1 (MQ=255) cACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTg < 1:194267/69‑1 (MQ=255) cACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTg < 1:2149821/69‑1 (MQ=255) cACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTg < 1:631823/69‑1 (MQ=255) cACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTg < 1:2924793/69‑1 (MQ=255) cACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTg < 1:3009143/69‑1 (MQ=255) aCGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTg < 1:3347096/68‑1 (MQ=255) aCGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTg < 1:270634/68‑1 (MQ=255) gAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCt < 1:2535245/69‑1 (MQ=255) gAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCt < 1:812496/69‑1 (MQ=255) aaGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCt < 1:1773862/68‑1 (MQ=255) gggTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGa < 1:376127/68‑1 (MQ=255) ggTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTgatgat < 1:696140/47‑1 (MQ=255) gTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACtt > 1:116209/1‑69 (MQ=255) ccAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTg < 1:567177/52‑1 (MQ=255) ggTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGa < 1:2960725/69‑1 (MQ=255) ggTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGa < 1:2956685/69‑1 (MQ=255) cAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAggg > 1:3075537/1‑65 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGa > 1:623850/1‑59 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGaa > 1:1848832/1‑69 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGaa > 1:1373352/1‑69 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGaa > 1:972593/1‑69 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGaa > 1:1248801/1‑69 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGaa > 1:501211/1‑69 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGaa > 1:3179574/1‑69 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGaa > 1:3125599/1‑69 (MQ=255) gcgcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCAGACTTGATCAGGGAAGTGGGGGaa > 1:1612862/1‑69 (MQ=255) gcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTTATCAGGGAAGTGGGGGAAGc > 1:2551295/1‑69 (MQ=255) gcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTggg > 1:866185/1‑63 (MQ=255) gcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGTGAAGc > 1:3382032/1‑69 (MQ=255) gcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGc > 1:471638/1‑69 (MQ=255) gcATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGc > 1:1694941/1‑69 (MQ=255) aTCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTc < 1:2031886/69‑1 (MQ=255) tCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTc < 1:542090/68‑1 (MQ=255) tCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTc < 1:1911666/68‑1 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGAttt > 1:1200013/1‑36 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCa > 1:323194/1‑48 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAg > 1:2776399/1‑49 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAg > 1:2235375/1‑49 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAg > 1:1552163/1‑49 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAg > 1:1984875/1‑49 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAg > 1:1803341/1‑49 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTc > 1:2375033/1‑69 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTc > 1:68739/1‑69 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTc > 1:1269821/1‑69 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTc > 1:587405/1‑69 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTc > 1:2992506/1‑69 (MQ=255) ggCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTc > 1:2837203/1‑69 (MQ=255) tATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTCAGGTGGACAt < 1:3110518/69‑1 (MQ=255) tATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTCAGGTGGACAt < 1:873695/69‑1 (MQ=255) tATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTCAGGTGGACAt < 1:2794706/69‑1 (MQ=255) tATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTCAGGTGGACAt < 1:2784488/69‑1 (MQ=255) tATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTCAGGTGGACAt < 1:1012388/69‑1 (MQ=255) | AGTTAGTGAACGTGGAAGAGCACGTTGAAGGGTTCCGCCAGGTCAGAGAGGCGCATCGGCGCGAGCTTATTGATGATTACGTTGAGCTGATTTCTGACTTGATCAGGGAAGTGGGGGAAGCTCGTCAGGTGGACAT > minE/544143‑544278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |