| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 547,857 | C→T | 74.5% | T326T (ACC→ACT) | ybiT → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 547,857 | 0 | C | T | 74.5% | 41.4 / 11.5 | 39 | T326T (ACC→ACT) | ybiT | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (8/2); new base T (16/13); total (24/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGGAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAA > minE/547797‑547920 | cATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGGAAGTGGAAGGTCTGACCAAAGGGt > 1:2076466/1‑68 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:1057309/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:3355555/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:2442444/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:2364620/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:390739/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:2203198/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:2184882/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:515707/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:2034428/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:3283077/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:1950244/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:667554/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:2836846/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAcc > 1:1461993/1‑69 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAc > 1:1995945/1‑68 (MQ=255) gTAACGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGATAACGGTCCACTGTTTAAAAATCTCAAc > 1:449708/1‑68 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:2642182/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:3123814/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:3325716/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:594788/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:87744/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:966783/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:3087867/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:266795/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:2611804/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:2590460/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:2199435/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:217439/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACTAAAGGGTTTGAt < 1:1235999/38‑1 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACctgc > 1:199530/1‑69 (MQ=255) aCGCGCTGGAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACctgc > 1:1660589/1‑69 (MQ=255) gTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGaa > 1:659397/1‑68 (MQ=255) cTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGaaaaa > 1:3343529/1‑69 (MQ=255) cTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGaaaaa > 1:374811/1‑69 (MQ=255) cTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGaaaaa > 1:233361/1‑69 (MQ=255) cTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGaaaaa > 1:1715353/1‑69 (MQ=255) cTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGaaaa < 1:3142477/68‑1 (MQ=255) cTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGaaaa < 1:826977/68‑1 (MQ=255) | CATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGGAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAA > minE/547797‑547920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |