| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 548,043 | T→C | 37.7% | A388A (GCT→GCC) | ybiT → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 548,043 | 0 | T | C | 37.7% | 27.8 / 32.1 | 53 | A388A (GCT→GCC) | ybiT | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base T (25/8); new base C (7/13); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
CTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAG > minE/547984‑548100 | cTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCa > 1:2477657/1‑68 (MQ=255) aCCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAat > 1:2711408/1‑68 (MQ=255) aCCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAat > 1:996400/1‑68 (MQ=255) aCCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAat > 1:907626/1‑68 (MQ=255) aCCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAat > 1:2386573/1‑68 (MQ=255) aCCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAat > 1:2332840/1‑68 (MQ=255) aCCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAat > 1:2199537/1‑68 (MQ=255) aCCGGACAGCGGCACCGTAAAATGGGCTGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGaa > 1:1777992/1‑67 (MQ=255) ggCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGaa < 1:1578945/69‑1 (MQ=255) gCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGaa < 1:147478/68‑1 (MQ=255) cGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGa < 1:565340/69‑1 (MQ=255) cTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCgtg < 1:2432672/68‑1 (MQ=255) cTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCgtg < 1:2819744/68‑1 (MQ=255) cTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCgtg < 1:1041959/68‑1 (MQ=255) cTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCgtg < 1:2507351/68‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:2224282/52‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:1515548/52‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:685610/52‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:3363391/52‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:1840407/52‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:2673709/52‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:526454/52‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:496673/52‑1 (MQ=255) tGAGAACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:946176/52‑1 (MQ=255) agaACGCGCGCATTGGTTACTACGCCCAGTACCATGAATATGAGTTCGaa < 1:2755516/50‑1 (MQ=25) agaACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:153281/50‑1 (MQ=255) agaACGCGCGCATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:2842147/50‑1 (MQ=255) gcgcgcATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATgg < 1:804114/69‑1 (MQ=255) cgcgcATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATgg > 1:3216270/1‑68 (MQ=255) cgcgcATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATgg > 1:3177855/1‑68 (MQ=255) cgcgcATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATgg > 1:344198/1‑68 (MQ=255) cgcgcATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATgg > 1:1397716/1‑68 (MQ=255) cgcgcATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATgg > 1:2336788/1‑68 (MQ=255) cgcgcATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATgg > 1:583532/1‑68 (MQ=255) cATTGGTTACTACGCCCAGGACCATGAATATGAGTTCGaa < 1:1722129/40‑1 (MQ=255) ggTTACTATGCTCAGGACCACGAATATTAGTTTGAAAATGATCTGAcc > 1:1803526/1‑48 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCg > 1:2319037/1‑49 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:1223567/1‑69 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:449855/1‑69 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:2701799/1‑69 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:574017/1‑69 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:713353/1‑69 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:78299/1‑69 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:1817240/1‑69 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:1295238/1‑69 (MQ=255) ggTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAg > 1:91978/1‑69 (MQ=255) cTCAGGACCATGAATATGAGTTTGAAAATGATCTGACCGTGTTCg > 1:234811/1‑45 (MQ=255) cTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCg > 1:3097152/1‑45 (MQ=255) cTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCg > 1:977920/1‑45 (MQ=255) cTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCg > 1:939211/1‑45 (MQ=255) cTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCg > 1:2709711/1‑45 (MQ=255) cTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCg > 1:303606/1‑45 (MQ=255) cTCAGGACAACGAATATGAGTTTGAAAATGATCTGACCGTGTTCg > 1:3335119/1‑45 (MQ=255) atCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCg > 1:1967808/2‑45 (MQ=255) | CTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGTTCGAATGGATGAGCCAG > minE/547984‑548100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |