| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 551,567 | C→T | 30.6% | V653I (GTT→ATT) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 551,567 | 0 | C | T | 30.6% | 114.1 / 55.1 | 117 | V653I (GTT→ATT) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base C (36/45); new base T (18/18); total (54/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 117 total aligned reads displayed.
GTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGC > minE/551503‑551630 | gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCgg > 1:2676591/1‑68 (MQ=255)gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCgg > 1:900834/1‑68 (MQ=255)gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCgg > 1:2883100/1‑68 (MQ=255)gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCgg > 1:437097/1‑68 (MQ=255)gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCgg > 1:3420962/1‑68 (MQ=255)gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCgg > 1:3305839/1‑68 (MQ=255) tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGAcc > 1:2364032/1‑68 (MQ=255) tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGAcc > 1:2446158/1‑68 (MQ=255) tGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGAcc > 1:261067/1‑68 (MQ=255) tGCGCGCCAAACGGTACGTTAGCGGAGATTGAAGACGTACCCGCGTAATAGTTGCCGCCAACCGGAc > 1:3254541/1‑67 (MQ=255) cgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGAc < 1:1665900/69‑1 (MQ=255) cgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGAc < 1:1917496/69‑1 (MQ=255) cgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGAc < 1:20953/69‑1 (MQ=255) cgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGAc < 1:2785347/69‑1 (MQ=255) cgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGAc < 1:3354407/69‑1 (MQ=255) aCGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAgc > 1:1621764/1‑69 (MQ=255) aCGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAgc > 1:428656/1‑69 (MQ=255) aCGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACAGTAgc > 1:2149288/1‑69 (MQ=255) gTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGAc < 1:1140016/56‑1 (MQ=255) ttAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTa > 1:856210/1‑69 (MQ=255) ttAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTa > 1:55803/1‑69 (MQ=255) gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:697927/69‑1 (MQ=255) gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:1130763/69‑1 (MQ=255) aCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:801262/68‑1 (MQ=255) tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcg > 1:2664738/1‑68 (MQ=255) aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc < 1:1754245/69‑1 (MQ=255) aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc < 1:2879422/69‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATg > 1:2817336/1‑48 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:3251052/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:2630074/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:308712/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:308617/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2274804/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2727031/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2968801/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2756524/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1040270/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:468607/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:889548/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:877861/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:746327/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1263146/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1380783/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1381863/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:1447333/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:632148/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1823702/1‑68 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGatt < 1:1618396/69‑2 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1612113/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2647858/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2675630/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1433513/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1667197/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1811461/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:656589/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:239740/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:3275337/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1139952/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:384010/67‑1 (MQ=255) gTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:3172423/65‑1 (MQ=255) aaTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2842351/63‑1 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:962830/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:2868709/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:1591219/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:1358796/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:2248421/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2505662/64‑1 (MQ=255) aGTTCCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2034816/60‑1 (MQ=255) gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:1218173/69‑1 (MQ=255) gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:1240856/69‑1 (MQ=255) gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:3133001/69‑1 (MQ=255) gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:788999/69‑1 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:1882143/1‑68 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:3076916/1‑68 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1190083/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1154880/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2940394/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1358155/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:3401075/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2627800/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:3156883/69‑1 (MQ=255) ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2086929/68‑1 (MQ=255) ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1243987/68‑1 (MQ=255) ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1859989/68‑1 (MQ=255) cAATCGGACCACGCCCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:373391/1‑67 (MQ=255) cAATCGGACCACGCCCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:639406/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1256445/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1107120/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:820418/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1972055/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2095337/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2328452/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:3182747/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2659977/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2678056/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2692347/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2751404/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2787839/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2795191/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAg > 1:2354645/1‑66 (MQ=255) | GTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGC > minE/551503‑551630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |