| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 551,589 | A→G | 34.1% | N645N (AAT→AAC) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 551,589 | 0 | A | G | 34.1% | 84.5 / 67.3 | 117 | N645N (AAT→AAC) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base A (24/53); new base G (18/22); total (42/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
TTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTA > minE/551524‑551646 | ttAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTa > 1:55803/1‑69 (MQ=255)ttAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTa > 1:856210/1‑69 (MQ=255) gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:697927/69‑1 (MQ=255) gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:1130763/69‑1 (MQ=255) aCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:801262/68‑1 (MQ=255) tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcg > 1:2664738/1‑68 (MQ=255) aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc < 1:2879422/69‑1 (MQ=255) aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc < 1:1754245/69‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATg > 1:2817336/1‑48 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1040270/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:1447333/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2727031/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:308712/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:2650064/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:241519/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:632148/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:468607/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2274804/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1380783/1‑68 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:3251052/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:889548/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2756524/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:308617/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2968801/68‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:877861/68‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGatt < 1:1618396/69‑2 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1811461/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1139952/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:239740/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2647858/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:3275337/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:656589/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2675630/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1667197/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1520178/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1433513/69‑1 (MQ=255) gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:384010/67‑1 (MQ=255) gTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:3172423/65‑1 (MQ=255) aaTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2842351/63‑1 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:2868709/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:962830/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:2248421/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:1358796/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:1591219/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:1726306/1‑43 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2505662/64‑1 (MQ=255) aGTTCCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2034816/60‑1 (MQ=255) gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:1218173/69‑1 (MQ=255) gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:1240856/69‑1 (MQ=255) gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:788999/69‑1 (MQ=255) gTTGCCGCCAATCGGACCACGACCGTAGCGCGCGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:425944/69‑1 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:1882143/1‑68 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:3076916/1‑68 (MQ=255) tGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:644013/57‑1 (MQ=255) ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1263993/57‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2627800/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1358155/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2940394/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1190083/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:3156883/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2682453/69‑1 (MQ=255) gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1154880/69‑1 (MQ=255) ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1919200/68‑1 (MQ=255) ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1859989/68‑1 (MQ=255) ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1243987/68‑1 (MQ=255) ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2086929/68‑1 (MQ=255) cAATCGGACCACGCCCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:373391/1‑67 (MQ=255) cAATCGGACCACGCCCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:639406/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:3182747/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:820418/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2659977/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1107120/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2795191/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2751404/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1256445/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2692347/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1972055/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2095337/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2189440/1‑67 (MQ=255) cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAg > 1:2354645/1‑66 (MQ=255) cGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:3175431/57‑1 (MQ=255) ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGcc > 1:1891343/1‑69 (MQ=255) ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGcc > 1:1276452/1‑69 (MQ=255) ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGcc > 1:2342072/1‑69 (MQ=255) gACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1432224/61‑1 (MQ=255) aCGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:3062475/57‑1 (MQ=255) aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag < 1:76777/69‑1 (MQ=255) aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGAcagcag < 1:1265130/69‑1 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1768009/42‑1 (MQ=255) aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCga < 1:1142452/69‑2 (MQ=255) aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:719720/69‑1 (MQ=255) aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:489835/69‑1 (MQ=255) aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:2728597/69‑1 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:2242289/69‑1 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:780962/69‑1 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:1405549/69‑1 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:1191192/69‑1 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:905594/69‑1 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTATGATAAGCGCGAGCCAGCAGCGTa < 1:785086/69‑1 (MQ=255) cGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGtta < 1:2861693/38‑1 (MQ=255) | TTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTA > minE/551524‑551646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |