| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 552,615 | G→C | 27.6% | L303L (CTC→CTG) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 552,615 | 0 | G | C | 27.6% | 185.1 / 77.4 | 130 | L303L (CTC→CTG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (38/56); new base C (24/12); total (62/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
CTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGAT > minE/552548‑552672 | cTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTg < 1:1182388/68‑1 (MQ=255)cTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTg < 1:3193563/68‑1 (MQ=255)cTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTg < 1:272147/68‑1 (MQ=255)cTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTg < 1:2208768/68‑1 (MQ=255) gccagcAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:620428/1‑69 (MQ=255) gccagcAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1863799/1‑69 (MQ=255) gccagcAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1077876/1‑69 (MQ=255) gccagcAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1475864/1‑69 (MQ=255) gccagcAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:2522456/1‑69 (MQ=255) cagcagCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGa < 1:2482425/69‑1 (MQ=255) gcTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:1692904/69‑1 (MQ=255) gcTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa > 1:1046834/1‑69 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:1667705/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:2034262/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:2384340/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:1310733/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:3013567/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:519068/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:52555/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:65499/69‑1 (MQ=255) tATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:104371/68‑1 (MQ=255) aTGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAg < 1:2179043/64‑1 (MQ=255) tGCAGCATCTCGATGGCGTGTTCGCGATCGAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:3424823/66‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGt > 1:2116811/1‑62 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2699647/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2722611/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2819501/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2478387/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2928884/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:121731/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:3176891/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:1183604/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:3270544/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:1510761/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:3043837/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:2919035/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:796820/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:2772418/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:360069/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:1858812/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:3068446/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:1884032/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2039974/69‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:336475/69‑1 (MQ=255) agcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2187334/68‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:1519392/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:972178/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:2733872/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:897587/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:2902357/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1030900/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:2955745/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:2964868/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:2996911/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:3013871/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:3113074/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:1293681/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1956185/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:2010344/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:2067243/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:1930010/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:2410243/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:139428/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1515496/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:2617680/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga < 1:2266969/69‑1 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATagag > 1:1814285/1‑59 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGAt > 1:3182202/1‑61 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTgtt > 1:2258843/1‑65 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:731751/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:677928/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:753528/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:521025/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:111101/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1859705/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:771867/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3270680/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:987987/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1458542/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:2672397/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:285360/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1404161/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3051598/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1431766/1‑68 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGTTACTGGTCCATAc < 1:1059807/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:3220422/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:92417/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:1761593/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2104811/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:25562/69‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:570488/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2468129/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:680714/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2540282/68‑1 (MQ=255) ttCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1301486/69‑1 (MQ=255) ttCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1737838/69‑1 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1570941/1‑68 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:2320135/1‑68 (MQ=255) cgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAg < 1:1053933/39‑1 (MQ=255) gCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGAt < 1:825260/68‑1 (MQ=255) | CTTCCAGCAGTTTCAGCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGAT > minE/552548‑552672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |