| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 552,627 | G→A | 36.6% | R299R (CGC→CGT) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 552,627 | 0 | G | A | 36.6% | 63.8 / 59.3 | 98 | R299R (CGC→CGT) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (19/43); new base A (24/12); total (43/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.13e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 139 total aligned reads displayed.
GCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGA > minE/552563‑552694 | gccagcAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1475864/1‑69 (MQ=255)gccagcAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1863799/1‑69 (MQ=255)gccagcAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1077876/1‑69 (MQ=255) cagcagCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGa < 1:2482425/69‑1 (MQ=255) gcTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:1692904/69‑1 (MQ=255) gcTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa > 1:1046834/1‑69 (MQ=255) gcTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2520088/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:1667705/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:1967663/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:2034262/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:1310733/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:2343482/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:2384340/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:3013567/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:519068/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:52555/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:65499/69‑1 (MQ=255) cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:65848/69‑1 (MQ=255) tATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:104371/68‑1 (MQ=255) aTGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAg < 1:2179043/64‑1 (MQ=255) tGCAGCATCTCGATGGCGTGTTCGCGATCGAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:3424823/66‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGt > 1:2116811/1‑62 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2722611/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:3270544/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2478387/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:121731/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2699647/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2819501/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:1183604/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTa < 1:2928884/63‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:1858812/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:3043837/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:2772418/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:360069/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:3068446/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:2919035/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:796820/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:732180/69‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:336475/69‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2039974/69‑1 (MQ=255) agcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2187334/68‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:2617680/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:3113074/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:2902357/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:3008872/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:2733872/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:2955745/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:2996911/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:1293681/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:1519392/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:972178/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1515496/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:897587/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc < 1:1930010/52‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1956185/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:1030900/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:2067243/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCgcggc > 1:43385/1‑52 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga < 1:311646/69‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga < 1:2266969/69‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTATGGTTGAGTTCAACGTCgcggc < 1:1481955/52‑1 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATagag > 1:1814285/1‑59 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGAt > 1:3182202/1‑61 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTgtt > 1:2258843/1‑65 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:111101/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:731751/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:771867/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3270680/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:987987/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:285360/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1431766/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1878207/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1412836/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1404161/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1306149/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:2672397/1‑68 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGATATACTGGTCCATAc < 1:557936/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGTTACTGGTCCATAc < 1:1059807/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:1761593/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:3220422/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:92417/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2251906/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2104811/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:25562/69‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2468129/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2540282/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:680714/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:570488/68‑1 (MQ=255) ttCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1737838/69‑1 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:2320135/1‑68 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:3048753/1‑68 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1570941/1‑68 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1857697/1‑68 (MQ=255) cgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAg < 1:1053933/39‑1 (MQ=255) gCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGAt < 1:825260/68‑1 (MQ=255) cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGa < 1:3181928/52‑1 (MQ=255) cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGa < 1:1553478/52‑1 (MQ=255) cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGAc > 1:2423510/1‑63 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:2949840/69‑1 (MQ=255) gcggcgATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:1196037/68‑1 (MQ=255) | GCCAGCAGCTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGA > minE/552563‑552694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |