| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 552,639 | C→T | 58.4% | P295P (CCG→CCA) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 552,639 | 0 | C | T | 58.4% | 26.5 / 62.2 | 84 | P295P (CCG→CCA) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base C (15/20); new base T (24/25); total (39/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCA > minE/552571‑552706 | cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:3292406/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:519068/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:1967663/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:52555/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:2343482/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:2384340/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:2034262/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:65499/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:65848/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:1667705/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:1310733/69‑1 (MQ=255)cTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:3013567/69‑1 (MQ=255) tATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:104371/68‑1 (MQ=255) tGCAGCATCTCGATGGCGTGTTCGCGATCGAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTAc < 1:3424823/66‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:3068446/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:2772418/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:360069/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:3043837/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:2919035/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:1858812/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:1884032/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:796820/1‑67 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2039974/69‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2874359/69‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:336475/69‑1 (MQ=255) cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:732180/69‑1 (MQ=255) agcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2187334/68‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga < 1:2266969/69‑1 (MQ=255) aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga < 1:311646/69‑1 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATagag > 1:1814285/1‑59 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGAt > 1:3182202/1‑61 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTgtt > 1:2258843/1‑65 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3270680/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3215557/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3051598/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:2672397/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:2765119/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:285360/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:521025/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:987987/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:111101/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:771867/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:753528/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1306149/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1404161/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1412836/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1431766/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1458542/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:731751/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:677928/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1859705/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1878207/1‑68 (MQ=255) gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:2176948/1‑68 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGATATACTGGTCCATAc < 1:557936/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGTTACTGGTCCATAc < 1:1059807/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2104811/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:92417/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2251906/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:1761593/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:3220422/69‑1 (MQ=255) gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:25562/69‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:570488/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:680714/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2468129/68‑1 (MQ=255) ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2540282/68‑1 (MQ=255) ttCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1301486/69‑1 (MQ=255) ttCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1737838/69‑1 (MQ=255) tCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1080531/68‑1 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:3048753/1‑68 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1570941/1‑68 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:2320135/1‑68 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1191639/1‑68 (MQ=255) cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1857697/1‑68 (MQ=255) gCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGAt < 1:825260/68‑1 (MQ=255) cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGa < 1:3181928/52‑1 (MQ=255) cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGa < 1:1553478/52‑1 (MQ=255) cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGt > 1:2256545/1‑60 (MQ=255) cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGAc > 1:2423510/1‑63 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3369700/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2002041/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2015891/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:268360/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:281923/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3362800/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2955335/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2578753/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3044464/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2676572/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3029292/66‑1 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:406181/69‑1 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:2949840/69‑1 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:2306229/69‑1 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:1763854/69‑1 (MQ=255) cacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2948374/66‑1 (MQ=255) gcggcgATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:1196037/68‑1 (MQ=255) acggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2591301/66‑1 (MQ=255) aCGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCa < 1:950838/69‑1 (MQ=255) aCGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCa < 1:1888020/69‑1 (MQ=255) | CTATGCAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCA > minE/552571‑552706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |