| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 552,675 | T→C | 42.6% | S283S (TCA→TCG) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 552,675 | 0 | T | C | 42.6% | 31.4 / 83.6 | 108 | S283S (TCA→TCG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base T (40/22); new base C (16/30); total (56/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGC > minE/552620‑552729 | cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGt > 1:2256545/1‑60 (MQ=255)cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGAc > 1:2423510/1‑63 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:268360/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3369700/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2002041/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2015891/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3362800/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3044464/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3029292/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2955335/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2676572/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:281923/66‑1 (MQ=255) tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2578753/66‑1 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:2949840/69‑1 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:2306229/69‑1 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:406181/69‑1 (MQ=255) cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:1763854/69‑1 (MQ=255) cacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2948374/66‑1 (MQ=255) gcggcgATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:1196037/68‑1 (MQ=255) aCGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCa < 1:950838/69‑1 (MQ=255) aCGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCa < 1:1888020/69‑1 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg > 1:2208319/3‑53 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:2513221/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:3219208/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:3012177/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:296870/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:2420731/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:958896/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:1155044/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:1804718/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:1745328/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:3431826/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:389559/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:1582924/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:1390394/3‑68 (MQ=255) gatAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAAc > 1:561734/3‑68 (MQ=255) tAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:2958023/52‑1 (MQ=255) aCTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGt > 1:268799/1‑69 (MQ=255) aCTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGt > 1:596647/1‑69 (MQ=255) aCTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGt > 1:1623187/1‑69 (MQ=255) aCTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGt > 1:3430254/1‑69 (MQ=255) ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:1479989/68‑1 (MQ=255) ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:126678/68‑1 (MQ=255) ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:1243625/68‑1 (MQ=255) ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:2350015/68‑1 (MQ=255) ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:2774433/68‑1 (MQ=255) ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:1155881/68‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:814707/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:3372594/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:1139838/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2737876/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:873739/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2853311/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:64477/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:426258/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2958077/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:262152/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:20421/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2528551/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:1260063/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2211186/69‑1 (MQ=255) cGACCACACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:561/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGc > 1:3250831/1‑39 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGGAAAATCAACTGGATGAAGTAACACAGTTGc > 1:402252/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACt > 1:1399323/1‑49 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATTAAGTAACACAGTTGc > 1:1141903/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1639856/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1999193/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:1603225/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1551017/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:152304/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:475243/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1431530/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:805144/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1225227/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:868577/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1191110/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1127211/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:976847/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:987803/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1867285/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:2747210/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2634458/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2548227/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2535605/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2475490/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2438399/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:238163/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2324609/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:2972266/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:3029120/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2205789/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:3039106/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2203189/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:2025790/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2716244/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1830233/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTACATTCGATCTGCAAAATCAACTGGATCaa > 1:2887397/1‑56 (MQ=255) cGACCAAACGATACTGAGTGACCGATAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2645767/1‑69 (MQ=255) cGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:44431/68‑1 (MQ=255) | CAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGC > minE/552620‑552729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |