| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 552,723 | C→T | 29.4% | L267L (CTG→CTA) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 552,723 | 0 | C | T | 29.4% | 100.5 / 42.4 | 98 | L267L (CTG→CTA) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base C (46/23); new base T (11/18); total (57/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCAT > minE/552656‑552788 | ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:2350015/68‑1 (MQ=255)ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:1155881/68‑1 (MQ=255)ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:2774433/68‑1 (MQ=255)ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:1479989/68‑1 (MQ=255)ccATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAacac < 1:126678/68‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2211186/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:20421/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2528551/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:262152/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2737876/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:1866910/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2853311/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:2958077/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:3372594/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:1428222/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:426258/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:873739/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:1260063/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:1139838/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:814707/69‑1 (MQ=255) aCGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:64477/69‑1 (MQ=255) cGACCACACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:561/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGGAAAATCAACTGGATGAAGTAACACAGTTGc > 1:402252/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATTAAGTAACACAGTTGc > 1:1141903/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:3074470/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:238163/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:231982/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2324609/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:976847/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:475243/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2438399/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2475490/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:868577/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2535605/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:2972266/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2634458/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2716244/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:3039106/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:2747210/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:805144/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:532351/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2935202/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:3029120/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2205789/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1830233/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1867285/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1639856/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1191110/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1225227/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:1603225/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1999193/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:2025790/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1127211/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc < 1:152304/69‑1 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2203189/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:1431530/1‑69 (MQ=255) cGACCAAACGATACTGAGTGACCGATAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGc > 1:2645767/1‑69 (MQ=255) cGACCAAACGATACCGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:44431/68‑1 (MQ=255) gAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACATAGTTg < 1:1106207/53‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:1913960/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:1303845/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:549992/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:2837200/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:3303787/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:2757365/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:1216876/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:118349/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:2126135/69‑1 (MQ=255) tGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc < 1:1326213/69‑1 (MQ=255) tCGATCTGCAAAATCAACTGGATGATGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:1999044/1‑69 (MQ=255) tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:1713322/1‑69 (MQ=255) tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:827635/1‑69 (MQ=255) tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:2998271/1‑69 (MQ=255) tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:1010836/1‑69 (MQ=255) tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:2111522/1‑69 (MQ=255) tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCtgg > 1:1447831/1‑69 (MQ=255) aTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTc > 1:1216204/1‑45 (MQ=255) aTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCg > 1:35446/1‑63 (MQ=255) aTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCg > 1:340104/1‑63 (MQ=255) aTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCg > 1:1445137/1‑63 (MQ=255) aTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCg > 1:2310493/1‑63 (MQ=255) aTCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCg > 1:1060677/1‑63 (MQ=255) tGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGttt < 1:1599156/68‑1 (MQ=255) tGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGttt < 1:2286310/68‑1 (MQ=255) tGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGttt < 1:1942216/68‑1 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTg > 1:192099/1‑68 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:395445/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:2407870/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:848026/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:2002836/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:2655936/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:1623584/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:2946290/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:3015197/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:306166/1‑69 (MQ=255) gTAACATAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGc > 1:1335272/1‑69 (MQ=255) acatAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCTAt > 1:2564765/1‑69 (MQ=255) acatAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCTAt > 1:338669/1‑69 (MQ=255) acacAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCAt > 1:389154/1‑69 (MQ=255) acacAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCAt > 1:2204733/1‑69 (MQ=255) | CCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGTTTTCTGCCAT > minE/552656‑552788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |