| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 553,997 | T→C | 40.9% | L153L (CTA→CTG) | ybiY ← | predicted pyruvate formate lyase activating enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 553,997 | 0 | T | C | 40.9% | 12.8 / 29.1 | 44 | L153L (CTA→CTG) | ybiY | predicted pyruvate formate lyase activating enzyme |
| Reads supporting (aligned to +/- strand): ref base T (9/17); new base C (8/10); total (17/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCC > minE/553929‑554056 | tatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGt < 1:720823/69‑1 (MQ=255)tatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGt < 1:2978445/69‑1 (MQ=255)tatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGt < 1:2665644/69‑1 (MQ=255)tatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGt < 1:2179227/69‑1 (MQ=255) cACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:944754/68‑1 (MQ=255) cACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:796589/68‑1 (MQ=255) cACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:724942/68‑1 (MQ=255) cACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:2456371/68‑1 (MQ=255) cACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:2339821/68‑1 (MQ=255) aCGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:2182003/67‑1 (MQ=255) aCGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:682752/67‑1 (MQ=255) aCGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:311202/67‑1 (MQ=255) aCGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:246454/67‑1 (MQ=255) aCGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc < 1:3099543/67‑1 (MQ=255) aGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCgg > 1:1570467/1‑67 (MQ=255) aGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc > 1:1040649/1‑68 (MQ=255) aGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc > 1:692743/1‑68 (MQ=255) aGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc > 1:517335/1‑68 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:2084235/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:652622/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:2799231/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:1236819/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:116816/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:1557900/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:803029/67‑1 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGc > 1:317294/1‑63 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca > 1:328482/1‑69 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca > 1:2412750/1‑69 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca > 1:2774543/1‑69 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca > 1:1912952/1‑69 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:3078550/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:3054992/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:1625375/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:3118498/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:3314223/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:915567/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:2895660/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:390170/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:647061/69‑1 (MQ=255) tCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:1694716/68‑1 (MQ=255) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:3384847/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:1342487/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:499818/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:3410239/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:2664145/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:2590993/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:289767/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:2630402/1‑69 (MQ=25) | TATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCC > minE/553929‑554056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |