| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 554,004 | A→G | 47.4% | M151T (ATG→ACG) | ybiY ← | predicted pyruvate formate lyase activating enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 554,004 | 0 | A | G | 47.4% | ‑2.3 / 31.0 | 38 | M151T (ATG→ACG) | ybiY | predicted pyruvate formate lyase activating enzyme |
| Reads supporting (aligned to +/- strand): ref base A (13/7); new base G (8/10); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
AGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGATC > minE/553951‑554068 | aGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCgg > 1:1570467/1‑67 (MQ=255)aGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc > 1:1040649/1‑68 (MQ=255)aGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc > 1:517335/1‑68 (MQ=255)aGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc > 1:692743/1‑68 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:116816/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:2799231/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:2084235/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:652622/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:1557900/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:803029/67‑1 (MQ=255) tttCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg < 1:1236819/67‑1 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGc > 1:317294/1‑63 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca > 1:1912952/1‑69 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca > 1:2412750/1‑69 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca > 1:328482/1‑69 (MQ=255) tGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca > 1:2774543/1‑69 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:647061/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:390170/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:3314223/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:915567/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:3118498/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:3078550/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:3054992/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:2895660/69‑1 (MQ=255) ctCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:1625375/69‑1 (MQ=255) tCGTGGCTGGCTTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGt < 1:1694716/68‑1 (MQ=255) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:289767/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:2664145/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:2630402/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:2590993/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:3384847/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:3410239/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:1342487/1‑69 (MQ=25) ggctTGCAGCAGCGCCGTCGCCATTTCCGGCTGCATAAACGGTTCACCGCCAGAAAGCGTTAAgccgcc > 1:499818/1‑69 (MQ=25) gcgcCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGAt > 1:1978631/1‑69 (MQ=255) gcgcCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGAt > 1:1336350/1‑69 (MQ=255) gcgcCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGAt > 1:541103/1‑69 (MQ=255) gcgcCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGAt > 1:1921113/1‑69 (MQ=255) gccatcgccatTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGAt > 1:2970276/1‑67 (MQ=255) gccatcgccatTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGATc > 1:1775444/1‑68 (MQ=255) gccatcgccatTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGATc > 1:3022680/1‑68 (MQ=255) | AGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGCCGCTGCGATC > minE/553951‑554068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |