| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 555,416 | T→G | 30.9% | M211L (ATG→CTG) | moeB ← | molybdopterin synthase sulfurylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 555,416 | 0 | T | G | 30.9% | 119.7 / 87.6 | 123 | M211L (ATG→CTG) | moeB | molybdopterin synthase sulfurylase |
| Reads supporting (aligned to +/- strand): ref base T (26/59); new base G (15/23); total (41/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TCATCGCATCGTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCATTACGCCTGCTTC > minE/555359‑555482 | tCATCGCATCGTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCt > 1:70715/1‑68 (MQ=255)tCATCGCATCGTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCt > 1:86332/1‑68 (MQ=255)tCATCGCATCGTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCt > 1:781236/1‑68 (MQ=255)tCATCGCATCGTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCt > 1:787306/1‑68 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc > 1:3112221/1‑69 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc < 1:698586/69‑1 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc > 1:1952244/1‑69 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc > 1:1948148/1‑69 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc > 1:545736/1‑69 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc < 1:1863835/69‑1 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc < 1:2490011/69‑1 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc > 1:168957/1‑69 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc > 1:793179/1‑69 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc < 1:3163338/69‑1 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc < 1:3002090/69‑1 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc > 1:3404106/1‑69 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc < 1:1356153/69‑1 (MQ=255) catcgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc < 1:2899440/69‑1 (MQ=255) cgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAt > 1:425577/1‑69 (MQ=255) cgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAt > 1:2147294/1‑69 (MQ=255) cgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAt > 1:1201135/1‑69 (MQ=255) cgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAt > 1:1294622/1‑69 (MQ=255) cgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAt > 1:2331384/1‑69 (MQ=255) cgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAt > 1:1547680/1‑69 (MQ=255) cgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAt > 1:1620866/1‑69 (MQ=255) cgcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAt > 1:1595102/1‑69 (MQ=255) gcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAtt > 1:2611240/1‑69 (MQ=255) gcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAtt > 1:19332/1‑69 (MQ=255) gcatcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCAtt > 1:76987/1‑69 (MQ=255) tcgTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTc < 1:1604117/62‑1 (MQ=255) aCATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGc < 1:2004936/68‑1 (MQ=255) aCGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTg < 1:799511/45‑1 (MQ=255) aCGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTg < 1:1634442/45‑1 (MQ=255) aCGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTg < 1:747899/45‑1 (MQ=255) gATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:3192774/69‑1 (MQ=255) gATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2929492/69‑1 (MQ=255) gATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2769596/69‑1 (MQ=255) gATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:1729392/69‑1 (MQ=255) gATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2200326/69‑1 (MQ=255) gATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:1968509/69‑1 (MQ=255) gATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTATGATCGCTTCCATTGCCTGCAACGAAca < 1:1805365/69‑2 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:2264259/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:1875622/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:111352/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:2532871/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:399132/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:3432234/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:897901/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:3289827/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:104171/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:2877454/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:844663/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTGACATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:501749/1‑68 (MQ=255) ttttTCCGCTGGCCGGTTTCCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAACCa > 1:2139088/1‑68 (MQ=255) tttCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAAt < 1:2870954/69‑1 (MQ=255) tttCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAAt < 1:3172683/69‑1 (MQ=255) tttCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAAt < 1:987202/69‑1 (MQ=255) tttCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAAt < 1:3258464/69‑1 (MQ=255) tttCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAAt < 1:588616/69‑1 (MQ=255) tttCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAAt < 1:653174/69‑1 (MQ=255) tttCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAAt < 1:2845527/69‑1 (MQ=255) cccGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTACCATTGCCTGCAACGAACCAAt < 1:1239965/66‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:3159540/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:903645/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:1185151/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:853574/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:802676/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:1463101/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:1549776/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2046455/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2124257/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2207309/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2225620/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2686898/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2813956/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:2819841/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:336964/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:3119993/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:3159575/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:3001369/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:3089570/49‑1 (MQ=255) tGCCATAACCTGCCAGCAGTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:300843/49‑1 (MQ=255) aTAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:2442364/69‑1 (MQ=255) aTAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:1916781/69‑1 (MQ=255) aTAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:1886636/69‑1 (MQ=255) aTAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:1499836/69‑1 (MQ=255) aTAACCTGCCAGCATTTTGATCGCTTCCATT‑CCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:865098/68‑1 (MQ=255) tAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:1079631/68‑1 (MQ=255) aCCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAAcc < 1:1577023/42‑1 (MQ=255) aCCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:1605620/66‑1 (MQ=255) aCCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:1333874/66‑1 (MQ=255) aCCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:715420/66‑1 (MQ=255) aCCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:3363587/66‑1 (MQ=255) aCCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:639127/66‑1 (MQ=255) aCCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:911460/66‑1 (MQ=255) aCCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCAt < 1:2274988/66‑1 (MQ=255) cATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCATTACGCCTGCTTc < 1:3190920/69‑1 (MQ=255) cATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCATTACGCCTGCTTc < 1:3060611/69‑1 (MQ=255) cATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCATTACGCCTGCTTc < 1:3171108/69‑1 (MQ=255) cATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCATTACGCCTGCTTc < 1:674134/69‑1 (MQ=255) | TCATCGCATCGTACATGACGATTTTCCCGCTGGCCGGTTTGCCATAACCTGCCAGCATTTTGATCGCTTCCATTGCCTGCAACGAACCAATTACGCCGATCAACGGTGCCATTACGCCTGCTTC > minE/555359‑555482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |